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Journal of Bioenergetics and Biomembranes

, Volume 45, Issue 3, pp 229–241 | Cite as

AMPK modulates glucose-sensing in insulin-secreting cells by altered phosphotransfer to KATP channels

  • Craig Beall
  • Kenneth R. Watterson
  • Rory J. McCrimmon
  • Michael L. J. Ashford
Open Access
Article

Abstract

Glucose-sensing (GS) behaviour in pancreatic β-cells is dependent on ATP-sensitive K+ channel (KATP) activity, which is controlled by the relative levels of the KATP ligands ATP and ADP, responsible for closing and opening KATP, respectively. However, the mechanism by which β-cells transfer energy status from mitochondria to KATP, and hence to altered electrical excitability and insulin secretion, is presently unclear. Recent work has demonstrated a critical role for AMP-activated protein kinase (AMPK) in GS behaviour of cells. Electrophysiological recordings, coupled with measurements of gene and protein expression were made from rat insulinoma cells to investigate whether AMPK activity regulates this energy transfer process. Using the whole-cell recording configuration with sufficient intracellular ATP to keep KATP closed, raised AMPK activity induced GS electrical behaviour. This effect was prevented by the AMPK inhibitor, compound C and required a phosphotransfer process. Indeed, high levels of intracellular phosphocreatine or the presence of the adenylate kinase (AK) inhibitor AP5A blocked this action of AMPK. Using conditions that maximised AMPK-induced KATP opening, there was a significant increase in AK1, AK2 and UCP2 mRNA expression. Thus we propose that KATP opening in response to lowered glucose concentration requires AMPK activity, perhaps in concert with increased AK and UCP2 to enable mitochondrial-derived ADP signals to be transferred to plasma membrane KATP by phosphotransfer cascades.

Keywords

AMPK KATP Glucose β-cell Adenylate kinase Creatine kinase UCP2 

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Copyright information

© The Author(s) 2013

Open AccessThis article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited.

Authors and Affiliations

  • Craig Beall
    • 1
  • Kenneth R. Watterson
    • 1
  • Rory J. McCrimmon
    • 1
  • Michael L. J. Ashford
    • 1
  1. 1.Division of Cardiovascular and Diabetes Medicine, Medical Research Institute, Ninewells Hospital and Medical SchoolUniversity of DundeeDundeeUK

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