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Dietary avocado oil supplementation attenuates the alterations induced by type I diabetes and oxidative stress in electron transfer at the complex II-complex III segment of the electron transport chain in rat kidney mitochondria

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Abstract

Impaired complex III activity and reactive oxygen species (ROS) generation in mitochondria have been identified as key events leading to renal damage during diabetes. Due to its high content of oleic acid and antioxidants, we aimed to test whether avocado oil may attenuate the alterations in electron transfer at complex III induced by diabetes by a mechanism related with increased resistance to lipid peroxidation. 90 days of avocado oil administration prevented the impairment in succinate-cytochrome c oxidoreductase activity caused by streptozotocin-induced diabetes in kidney mitochondria. This was associated with a protection against decreased electron transfer through high potential chain in complex III related to cytochromes c + c 1 loss. During Fe2+-induced oxidative stress, avocado oil improved the activities of complexes II and III and enhanced the protection conferred by a lipophilic antioxidant against damage by Fe2+. Avocado oil also decreased ROS generation in Fe2+-damaged mitochondria. Alterations in the ratio of C20:4/C18:2 fatty acids were observed in mitochondria from diabetic animals that not were corrected by avocado oil treatment, which yielded lower peroxidizability indexes only in diabetic mitochondria although avocado oil caused an augment in the total content of monounsaturated fatty acids. Moreover, a protective effect of avocado oil against lipid peroxidation was observed consistently only in control mitochondria. Since the beneficial effects of avocado oil in diabetic mitochondria were not related to increased resistance to lipid peroxidation, these effects were discussed in terms of the antioxidant activity of both C18:1 and the carotenoids reported to be contained in avocado oil.

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  1. Instituto de Investigaciones Químico-Biológicas, Universidad Michoacana de San Nicolás de Hidalgo, Edificio B-3 Ciudad Universitaria, Morelia, Mich., 58030, México

    Omar Ortiz-Avila, Carlos Alberto Sámano-García, Alfredo Saavedra-Molina & Christian Cortés-Rojo

  2. Facultad de Enfermería, Universidad Michoacana de San Nicolás de Hidalgo, Av. Ventura Puente 115, Morelia, Mich., 58000, México

    Elizabeth Calderón-Cortés

  3. Unidad de Biomedicina. Facultad de Estudios Superiores Iztacala, Universidad Nacional Autónoma de México, Av. de los Barrios No 1 Los Reyes Iztacala, Tlalnepantla, Edo. de México, 54090, México

    Ismael H. Pérez-Hernández & Ricardo Mejía-Zepeda

  4. Facultad de Medicina Dr. Ignacio Chávez, Universidad Michoacana de San Nicolás de Hidalgo, Av. Dr. Rafael Carrillo s/n., Morelia, Mich., 58020, México

    Alain R. Rodríguez-Orozco

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  1. Omar Ortiz-Avila
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Ortiz-Avila, O., Sámano-García, C.A., Calderón-Cortés, E. et al. Dietary avocado oil supplementation attenuates the alterations induced by type I diabetes and oxidative stress in electron transfer at the complex II-complex III segment of the electron transport chain in rat kidney mitochondria. J Bioenerg Biomembr 45, 271–287 (2013). https://doi.org/10.1007/s10863-013-9502-3

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