Journal of Bioenergetics and Biomembranes

, Volume 44, Issue 3, pp 309–315 | Cite as

Characterization of calcium, phosphate and peroxide interactions in activation of mitochondrial swelling using derivative of the swelling curves

  • Zdeněk Drahota
  • René Endlicher
  • Pavla Staňková
  • David Rychtrmoc
  • Marie Milerová
  • Zuzana ČervinkováEmail author


We describe a new method for the analysis of mitochondrial swelling curves. Using classical swelling curves, only the maximum extent of the swelling can be calculated in a numerical form. However, taking the derivative of the classical swelling curves enables the evaluation of two additional parameters of the swelling process in a numerical form, namely, the maximum swelling rate after the addition of the swelling inducer (as dA520/10 s) and the time (in sec) at which the maximum swelling rate after the addition of the swelling inducer is obtained. The use of these three parameters enables the better characterization of the swelling process as demonstrated by the evaluation of calcium and phosphate interactions in the opening of the mitochondrial permeability transition pore and by the characterization of the peroxide potentiating action.


Mitochondrial swelling Mitochondrial permeability transition pore Calcium, phosphate and peroxide interactions 


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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  • Zdeněk Drahota
    • 1
    • 2
  • René Endlicher
    • 2
    • 3
  • Pavla Staňková
    • 2
  • David Rychtrmoc
    • 2
  • Marie Milerová
    • 4
  • Zuzana Červinková
    • 2
    Email author
  1. 1.Institute of Physiology and Center for Applied GenomicsAcademy of Sciences of the Czech RepublicPragueCzech Republic
  2. 2.Department of PhysiologyCharles University in Prague, Faculty of Medicine in Hradec KrálovéHradec KrálovéCzech Republic
  3. 3.Department of Anatomy Charles University in PragueFaculty of Medicine in Hradec KrálovéHradec KrálovéCzech Republic
  4. 4.Institute of Physiology and Center for Cardiovascular ResearchAcademy of Sciences of the Czech RepublicPragueCzech Republic

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