IL-6 induces lipolysis and mitochondrial dysfunction, but does not affect insulin-mediated glucose transport in 3T3-L1 adipocytes

  • Chenbo Ji
  • Xiaohui Chen
  • Chunlin Gao
  • Liuhong Jiao
  • Jianguo Wang
  • Guangfeng Xu
  • Hailong Fu
  • Xirong Guo
  • Yaping Zhao
Article

Abstract

Interleukin-6 (IL-6) has emerged as an important cytokine involved in the regulation of metabolism. However, the role of IL-6 in the etiology of obesity and insulin resistance is not fully understood. Mitochondria are key organelles of energy metabolism, and there is growing evidence that mitochondrial dysfunction plays a crucial role in the pathogenesis of obesity-associated insulin resistance. In this study, we determined the direct effect of IL-6 on lipolysis in adipocytes, and the effects of IL-6 on mitochondrial function were investigated. We found that cells treated with IL-6 displayed fewer lipids and an elevated glycerol release rate. Further, IL-6 treatment led to decreased mitochondrial membrane potential, decreased cellular ATP production, and increased intracellular ROS levels. The mitochondria in IL-6-treated cells became swollen and hollow with reduced or missing cristae. However, insulin-stimulated glucose transport was unaltered. PGC-1α, NRF1, and mtTFA mRNA levels were markedly increased, and the mitochondrial contents were also increased. Our results demonstrate that IL-6 can exert a direct lipolytic effect and induce mitochondrial dysfunction. However, IL-6 did not affect insulin sensitivity in adipocytes in vitro. We deduce that in these cells, enhanced mitochondrial biogenesis might play a compensatory role in glucose transport.

Keywords

IL-6 Adipocytes Lipolysis Mitochondria Insulin resistance 

Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Chenbo Ji
    • 1
    • 2
  • Xiaohui Chen
    • 2
  • Chunlin Gao
    • 3
  • Liuhong Jiao
    • 4
  • Jianguo Wang
    • 4
  • Guangfeng Xu
    • 4
  • Hailong Fu
    • 4
  • Xirong Guo
    • 1
    • 2
  • Yaping Zhao
    • 4
  1. 1.Department of PediatricsNanjing Maternal and Child Health Hospital of Nanjing Medical UniversityNanjingChina
  2. 2.Institute of PediatricsNanjing Medical UniversityNanjingChina
  3. 3.Department of PediatricsJinling HospitalNanjingChina
  4. 4.Department of Clinical LaboratoryThe 82nd Hospital of the People’s Liberation ArmyHuaianChina

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