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Journal of Bioenergetics and Biomembranes

, Volume 42, Issue 3, pp 245–253 | Cite as

Effects of a high fat diet on liver mitochondria: increased ATP-sensitive K+ channel activity and reactive oxygen species generation

  • Ariel R. Cardoso
  • João Victor Cabral-Costa
  • Alicia J. Kowaltowski
Article

Abstract

High fat diets are extensively associated with health complications within the spectrum of the metabolic syndrome. Some of the most prevalent of these pathologies, often observed early in the development of high-fat dietary complications, are non-alcoholic fatty liver diseases. Mitochondrial bioenergetics and redox state changes are also widely associated with alterations within the metabolic syndrome. We investigated the mitochondrial effects of a high fat diet leading to non-alcoholic fatty liver disease in mice. We found that the diet does not substantially alter respiratory rates, ADP/O ratios or membrane potentials of isolated liver mitochondria. However, H2O2 release using different substrates and ATP-sensitive K+ transport activities are increased in mitochondria from animals on high fat diets. The increase in H2O2 release rates was observed with different respiratory substrates and was not altered by modulators of mitochondrial ATP-sensitive K+ channels, indicating it was not related to an observed increase in K+ transport. Altogether, we demonstrate that mitochondria from animals with diet-induced steatosis do not present significant bioenergetic changes, but display altered ion transport and increased oxidant generation. This is the first evidence, to our knowledge, that ATP-sensitive K+ transport in mitochondria can be modulated by diet.

Keywords

Steatosis Respiration Mitochondrial bioenergetics Mitochondrial ATP-sensitive potassium channels (mitoKATPReactive oxygen species (ROS) 

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Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Ariel R. Cardoso
    • 1
  • João Victor Cabral-Costa
    • 1
  • Alicia J. Kowaltowski
    • 1
    • 2
  1. 1.Departamento de Bioquímica, Instituto de QuímicaUniversidade de São PauloSão PauloBrazil
  2. 2.Cidade UniversitáriaSão PauloBrazil

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