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Journal of Bioenergetics and Biomembranes

, Volume 42, Issue 1, pp 79–84 | Cite as

Dipyridamole increases gap junction coupling in bovine GM-7373 aortic endothelial cells by a cAMP-protein kinase A dependent pathway

  • D. Begandt
  • W. Bintig
  • K. Oberheide
  • S. Schlie
  • A. NgezahayoEmail author
Article

Abstract

The scrape-loading/dye transfer technique was applied on the bovine aortic endothelial cell line GM-7373 to analyze the effects of the antithrombolytic drug dipyridamole on gap junction coupling in endothelial cells. We found that a cell treatment for 24 h with dipyridamole in therapeutically relevant concentrations (1–100 µM) increased gap junction coupling in a dose dependent manner. Similar to dipyridamole, forskolin as well as 8-Br-cAMP increased the gap junction coupling, while dibutyryl-cGMP (db-cGMP) did not affect the gap junction coupling of the GM-7373 endothelial cells. In parallel, a pharmacological inhibition of protein kinase A (PKA) with N-[2-(p-bromocinnamylamino)ethyl]-5-isoquinolinesulfonamide dihydrochloride (H-89), antagonised the action of dipyridamole on gap junction coupling. We propose that the observed dipyridamole induced increase in gap junction coupling in endothelial cells is related to a cAMP-PKA dependent phosphorylation pathway. The report shows that gap junction coupling in endothelial cells is a suitable therapeutic target for treatment of cardiovascular diseases.

Keywords

Dipyridamole Endothelial cells Gap junction Connexins Scrape loading cAMP PKA H-89 

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Copyright information

© Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • D. Begandt
    • 1
  • W. Bintig
    • 1
  • K. Oberheide
    • 1
  • S. Schlie
    • 1
  • A. Ngezahayo
    • 1
    Email author
  1. 1.Institute of BiophysicsLeibniz University HannoverHannoverGermany

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