Porin isoform 2 has a different localization in Drosophila melanogaster ovaries than porin 1

  • Valeria Specchia
  • Francesca Guarino
  • Angela Messina
  • Maria Pia Bozzetti
  • Vito De Pinto


Eukaryotic porins or VDACs are a class of transmembrane proteins mainly localized in the outer mitochondrial membrane, whose function is to allow the diffusion of metabolites between the cytosol and the mitochondrion. In Drosophila melanogaster, as in other organisms, a small family of genes encoding porins has been discovered from the sequence of the genome. It is of general interest to understand whether these genes represent functional entities or not, and whether their product is associated to any particular tissue. In previous work we reported about the transcriptional and translational analysis of porin 1 and porin 2, and we proposed the specific presence of the latter in spermatozoa of the fly. In this paper we performed real time RT-PCR quantification of porin 1 and porin 2 transcripts in germ cells of the fly. It indicates that Porin 1 is abundantly expressed in both male and female tissues; Porin 2 instead, is very abundant in testis and it is present in ovaries as well, but in a small amount. The immuno-histological stain of ovaries shows that Porin isoform 1 is selectively targeted to follicular cells while Porin isoform 2 is present in mitochondria of the epithelial sheath cells of the ovariole. The implications of the subcellular distribution of these porin isoforms and the specific localization in germ tissues are discussed.


Voltage dependent anion selective channel Porin Mitochondria Drosophila melanogaster Ovary Ovariole Follicular cells Real time RT-PCR 


  1. Aiello R, Messina A, Schiffler B, Benz R, Tasco G, Casadio R, De Pinto V (2004) J Biol Chem 279:25364–25373CrossRefGoogle Scholar
  2. Baker MA, Lane DJ, Ly JD, De Pinto V, Lawen A (2004) J Biol Chem 279:4811–4819CrossRefGoogle Scholar
  3. Báthori G, Parolini I, Szabó I, Tombola F, Messina A, Oliva M, Sargiacomo M, De Pinto V, Zoratti M (2000) J Bioenerg Biomembranes 32:79–89CrossRefGoogle Scholar
  4. Boiko T, Rasband MN, Levinson SR, Caldwell JH, Mandel G, Trimmer JS, Matthews G (2001) Neuron 30:91–104CrossRefGoogle Scholar
  5. Casadio R, Jacoboni I, Messina A, De Pinto V (2002) FEBS Lett 520:1–7CrossRefGoogle Scholar
  6. Cox RT, Spradling AC (2003) Development 130:1579–1590CrossRefGoogle Scholar
  7. De Pinto V, Accardi R, Aiello R, Guarino F, Tommasello M, Messina A, Tommasino M, Jacoboni I, Casadio R, Benz R, De Giorgi F, Ichas F, Baker M, Lawen A (2003) It J Biochem 52:17–24Google Scholar
  8. De Pinto V, Benz R, Caggese C, Palmieri F (1989) Biochim Biophys Acta 987:1–7CrossRefGoogle Scholar
  9. Drosophila Genome Consortium (2007) Nature 450:203–218Google Scholar
  10. Fissore RA, Longo F, Anderson E, Parys JB, Ducibella T (1999) Biol Reprod 60:49–57CrossRefGoogle Scholar
  11. Graham B, Craigen W (2005) Mol Genet Metab 85:308–317CrossRefGoogle Scholar
  12. Guarino F, Specchia V, Zapparoli G, Messina A, Aiello R, Bozzetti MP, De Pinto V (2006) Biochem Biophys Res Commun 146:665–670CrossRefGoogle Scholar
  13. Hinsch KD, De Pinto V, Aires VA, Schneider X, Messina A, Hinsch E (2004) J Biol Chem 279:15281–15288CrossRefGoogle Scholar
  14. Hudson AM, Petrella LN, Tanaka AJ, Cooley L (2008) Dev Biol 314:329–340CrossRefGoogle Scholar
  15. Junankar PR, Dulhunty AF, Curtis SM, Pace SM, Thinnes FP (1995) J Muscle Res Cell Motil 16:595–610CrossRefGoogle Scholar
  16. King RC (1970) Ovarian development in drosophila melanogaster. Academic, New YorkGoogle Scholar
  17. Kroemer G, Galluzzi L, Brenner C (2007) Physiol Rev 87:99–163CrossRefGoogle Scholar
  18. Larionov A, Krause A, Miller W (2005) BMC Bioinformatics 6:62–78CrossRefGoogle Scholar
  19. Lehmann R, Tautz D (1994) Methods in cell biology vol. 44, pp. 575–598, Academic, New YorkGoogle Scholar
  20. Lemasters JJ, Holmuhamedov E (2006) Biochim Biophys Acta 1762:181–190Google Scholar
  21. Linden M, Karlsson G (1996) Biochem Biophys Res Commun 218:833–836CrossRefGoogle Scholar
  22. Messina A, Neri M, Perosa F, Caggese C, Marino M, Caizzi R, De Pinto V (1996) FEBS Lett 384:9–13CrossRefGoogle Scholar
  23. Middleton CA, Nongthomba U, Parry K, Sweeney ST, Sparrow JC, Elliott CJ (2006) BMC Biol 4:17CrossRefGoogle Scholar
  24. Oliva M, De Pinto V, Barsanti P, Caggese C (2002) Mol Genet Genomics 267:746–756CrossRefGoogle Scholar
  25. Oliva M, Messina A, Ragone G, Caggese C, De Pinto V (1998) FEBS Lett 430:327–332CrossRefGoogle Scholar
  26. Ponchel F, Toomes C, Bransfield K, Leong FT, Douglas SH, Field SL, Bell SM, Combaret V, Puisieux A, Mighell AJ, Robinson PA, Inglehearn CF, Isaacs JD, Markham AF (2003) BMC Biotechnol 3:18–31CrossRefGoogle Scholar
  27. Rapaport D (2003) EMBO Reports 4:948–952CrossRefGoogle Scholar
  28. Rosero O, Hämmerle B, Tejedor FJ (1997) J Neurosci 17:5108–5118Google Scholar
  29. Rostovtseva T, Colombini M (1997) Biophys J 72:1954–1962CrossRefGoogle Scholar
  30. Sampson MJ, Decker WK, Beaudet AL, Ruitenbeek W, Armstrong D, Hicks MJ, Craigen WJ (2001) J Biol Chem 276:39206–39212CrossRefGoogle Scholar
  31. Shoshan-Barmatz V, Hadad N, Feng W, Shafir I, Orr I, Varsanyi M, Heilmeyer LM (1996) FEBS Lett 386:205–210CrossRefGoogle Scholar
  32. Steinacker P, Awni LA, Becker S, Cole T, Reymann S, Hesse D, Kratzin HD, Morris-Wortmann C, Schwarzer C, Thinnes FP, Hilschmann N (2000) Int J Biochem Cell Biol 32:225–234CrossRefGoogle Scholar
  33. Szabadkai G, Bianchi K, Várnai P, De Stefani D, Wieckowski MR, Cavagna D, Nagy AI, Balla T, Rizzuto R (2006) J Cell Biol 175:901–911CrossRefGoogle Scholar
  34. Wang L, Zhu Y, Guo X, Huo R, Ma X, Lin M, Zhou ZM, Sha JH (2005) J Mol Med 83:812–821CrossRefGoogle Scholar
  35. Young MJ, Bay DC, Hausner G, Court DA (2007) BMC Evol Biol 7:31CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2008

Authors and Affiliations

  • Valeria Specchia
    • 1
  • Francesca Guarino
    • 2
    • 3
  • Angela Messina
    • 2
    • 3
  • Maria Pia Bozzetti
    • 1
  • Vito De Pinto
    • 2
    • 3
  1. 1.Department of Biological and Environmental Sciences and Technologies (DiSTeBA)University of SalentoLecceItaly
  2. 2.Department of Chemical SciencesUniversity of CataniaCataniaItaly
  3. 3.Research UnitNational Institute for Biostructures and BiosystemsRomeItaly

Personalised recommendations