Journal of Bioenergetics and Biomembranes

, Volume 39, Issue 2, pp 195–202

Mitochondrial dysfunction in platelets and hippocampi of senescence-accelerated mice

  • Jie Xu
  • Chun Shi
  • Qi Li
  • Jiajia Wu
  • E. Lucy Forster
  • David T. Yew
Article

DOI: 10.1007/s10863-007-9077-y

Cite this article as:
Xu, J., Shi, C., Li, Q. et al. J Bioenerg Biomembr (2007) 39: 195. doi:10.1007/s10863-007-9077-y

Abstract

Senescence-accelerated mice (SAM) strains are useful models to understand the mechanisms of age-dependent degeneration. In this study, measurements of the mitochondrial membrane potential (Δψm) of platelets and the Adenosine 5-triphosphate (ATP) content of hippocampi and platelets were made, and platelet mitochondria were observed in SAMP8 (faster aging mice) and SAMR1 (aging resistant control mice) at 2, 6 and 9 months of age. In addition, an Aβ-induced (Amyloid beta-protein) damage model of platelets was established. After the addition of Aβ, the Δψm of platelets of SAMP8 at 1and 6 months of age were measured. We found that platelet Δψm, and hippocampal and platelet ATP content of SAMP8 mice decreased at a relatively early age compared with SAMR1. The platelets of 6 month-old SAMP8 showed a tolerance to Aβ-induced damages. These results suggest that mitochondrial dysfunction might be one of the mechanisms leading to age-associated degeneration in SAMP mice at an early age and the platelets could serve as a biomarker for detection of mitochondrial function and age related disease.

Keywords

Aging Adenosine 5-triphosphate Mitochondria Mitochondrial membrane potential Senescence-accelerated mice 

Abbreviations

AD

(Alzheimer’s disease)

ADP/O

(Adenosine diphosphate/oxygen)

ATP

(Adenosine 5-[tetrahydrogen] triphosphate)

(amyloid beta-protein)

BDS

(base deactivated silica)

BP

(Band-pass filter)

Complex I

(NADH dehydrogenase)

Complex III

(cytochrome c-oxidoreductase)

Cu,Zn-SOD 1-3

(Copper zinc superoxide dismutase)

DMSO

(Dimethyl sulfoxide)

DNA

(deoxyribonucleic acid)

EDTA

(ethylenediaminetetraacetic acid)

FL

(Fluorescence channel)

HPLC

(High performance liquid chromatography

JC-1

(5,5,6,6-tetrachloro-1, 1,3,3-tetraethylbenzimidazolcarbocyanine iodide)

Mn-SOD

(manganese superoxide dismutase)

NADH

(Nicotinamide adenine dinucleotide, reduced)

PRP

(platelet-rich plasma)

PMT

(PhotoMultiplier tube)

PBS

(Phosphate buffer solution)

Redox

(oxidation/ reduction reaction)

SAM

(senescence-accelerated mice)

SAMP

(strains of accelerated senescence-prone mice)

SAMP8

(a substrain of SAMP)

SAMR

(strains of accelerated senescence-resistant mice)

SAMR1

(one of the three substrains of SAMR mice)

SPSS

(Statistical package for the social sciences)

TPP

(tetraphenyl-phosphonium)

8-OHG

(8-hydroxyguanosine)

Δψm

(mitochondrial membrane potential)

Copyright information

© Springer Science+Business Media, LLC 2007

Authors and Affiliations

  • Jie Xu
    • 1
  • Chun Shi
    • 1
  • Qi Li
    • 2
  • Jiajia Wu
    • 1
  • E. Lucy Forster
    • 2
  • David T. Yew
    • 2
  1. 1.Department of Anatomy, Zhongshan School of MedicineSun Yat-Sen University GuangzhouGuangdongChina
  2. 2.Department of AnatomyThe Chinese University of Hong KongShatinNew Territories Hong Kong

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