PRUNE and NM23-M1 expression in embryonic and adult mouse brain

  • Pietro Carotenuto
  • Natascia Marino
  • Anna Maria Bello
  • Anna D’Angelo
  • Umberto Di Porzio
  • Daniela Lombardi
  • Massimo Zollo
Original Paper

Abstract

A genetic interaction between PRUNE and NM23/NDPK has been postulated in Drosophila melanogaster. Many have focused on Drosophila for the genetic combination between PRUNE “knock down”and AWD/NM23 fly mutants bearing the P97S mutation (K-pn, Killer of PRUNE mutation). We postulated a role for PRUNE-NM23 interactions in vertebrate development, demonstrating a physical interaction between the human PRUNE and NM23-H1 proteins, and partially characterizing their functional significance in cancer progression. Here, we present an initial analysis towards the functional characterization of the PRUNE-NM23 interaction during mammalian embryogenesis. Our working hypothesis is that PRUNE, NM23-H1 and their protein-protein interaction partners have important roles in mammalian brain development and adult brain function. Detailed expression analyses from early mouse brain development to adulthood show significant co-expression of these two genes during embryonic stages of brain development, especially focusing on the cortex, hippocampus, midbrain and cerebellum. We hypothesize that their abnormal expression results in an altered pathway of activation, influencing protein complex formation and its protein partner interactions in early embryogenesis. In the adult brain, their function appears concentrated towards their enzyme activities, wherein biochemical variations can result in brain dysfunction.

Keywords

PDE cAMP NDPK Neurogenesis Differentiation Proliferation Brain development PRUNE NM23 

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Copyright information

© Springer Science+Business Media, Inc. 2006

Authors and Affiliations

  • Pietro Carotenuto
    • 1
  • Natascia Marino
    • 1
  • Anna Maria Bello
    • 1
  • Anna D’Angelo
    • 1
  • Umberto Di Porzio
    • 3
  • Daniela Lombardi
    • 4
  • Massimo Zollo
    • 1
    • 2
  1. 1.CEINGEBiotecnologie Avanzate ScarlNaplesItaly
  2. 2.Dipartimento di Biochimica e Biotecnologie MedicheUniversitá degli Studi di Napoli “Federico II”NaplesItaly
  3. 3.IGBInstitute of Genetics and Biophysics “Adriano Buzzati-Traverso,”, CNRNaplesItaly
  4. 4.Department of Experimental MedicineUniversity of L’AquilaL’AquilaItaly

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