Conformationally locked lanthanide chelating tags for convenient pseudocontact shift protein nuclear magnetic resonance spectroscopy
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Pseudocontact shifts (PCS) generated by lanthanide chelating tags yield valuable restraints for investigating protein structures, dynamics and interactions in solution. In this work, dysprosium-, thulium- and terbium-complexes of eight-fold methylated 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid tags [DOTA-M8-(4R4S)-SSPy] are presented that induce large pseudocontact shifts up to 5.5 ppm and adopt exclusively the square antiprismatic conformation. This is in contrast to our earlier findings on complexes of the stereoisomeric DOTA-M8-(8S)-SSPy, where significant amounts of the twisted square antiprismatic conformer for the Dy tag were observed. The Dy-, Tm-, Tb- and Lu-complexes of DOTA-M8-(4R4S)-SSPy were conjugated to ubiquitin S57C and selectively 15N leucine labeled human carbonic anhydrase II S50C, resulting in only one set of signals. Furthermore, we investigated the conformation of the thulium- and dysprosium-complexes in vacuo and with implicit water solvent using density functional theory calculations. The calculated energy differences between the two different conformations (7.0–50.5 kJ/mol) and experimental evidence from the corresponding ytterbium- and yttrium-complexes clearly suggest a SAP [Λ(δδδδ)] geometry for the complexes presented in this study. The lanthanide chelating tag studied in this work offer insights into the solution structure of proteins by inducing strong pseudocontact shifts, show different tensor properties compared to its predecessor, enables a convenient assignment procedure, is accessed by a more economic synthesis than its predecessor and constitutes a highly promising starting point for further developments of lanthanide chelating tags.
KeywordsNuclear magnetic resonance Pseudocontact shift Lanthanide chelating tag Paramagnetic Density functional theory Protein
The Chemistry Department of the University of Basel and the Swiss National Science Foundation Grant 200021_130263 are acknowledged for financial support. Calculations were performed at sciCORE (http://scicore.unibas.ch/) scientific computing core facility at University of Basel. Biological structures were generated using the open source software PyMOL (http://www.pymol.org/). C.E. Housecroft, E.C. Constable, T. Müntener and R. Vogel are acknowledged for helpful discussions. R.A. Byrd is gratefully acknowledged for a gift of M4-cyclen.
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Conflict of interest
The authors declare that they have no conflict of interest.
- Brewer KD, Bacaj T, Cavalli A, Camilloni C, Swarbrick JD, Liu J, Zhou A, Zhou P, Barlow N, Xu J, Seven AB, Prinslow EA, Voleti R, Häussinger D, Bonvin AM, Tomchick DR, Vendruscolo M, Graham B, Südhof TC, Rizo J (2015) Dynamic binding mode of a Synaptotagmin-1-SNARE complex in solution. Nat Struct Mol Biol 22:555–564CrossRefGoogle Scholar
- Natrajan LS, Khoabane NM, Dadds BL, Muryn CA, Pritchard RG, Heath SL, Kenwright AM, Kuprov I, Faulkner S (2010) Probing the structure, conformation, and stereochemical exchange in a family of lanthanide complexes derived from tetrapyridyl-appended cyclen. Inorg Chem 49:7700–7709CrossRefGoogle Scholar
- Ranganathan RS, Pillai RK, Raju N, Fan H, Nguyen H, Tweedle MF, Desreux JF, Jacques V (2002a) Polymethylated DOTA ligands. 1. Synthesis of rigidified ligands and studies on the effects of alkyl substitution on acid-base properties and conformational mobility. Inorg Chem 41:6846–6855CrossRefGoogle Scholar
- Regueiro-Figueroa M, Bensenane B, Ruscsák E, Esteban-Gómez D, Charbonnière LJ, Tircsó G, Tóth I, Blas AD, Rodríguez-Blas T, Platas-Iglesias C (2011) Lanthanide DOTA-like complexes containing a picolinate pendant: structural entry for the design of LnIII-based luminescent probes. Inorg Chem 50:4125–4141CrossRefGoogle Scholar