Journal of Biomolecular NMR

, Volume 62, Issue 4, pp 453–471 | Cite as

Combined automated NOE assignment and structure calculation with CYANA

  • Peter GüntertEmail author
  • Lena Buchner


The automated assignment of NOESY cross peaks has become a fundamental technique for NMR protein structure analysis. A widely used algorithm for this purpose is implemented in the program CYANA. It has been used for a large number of structure determinations of proteins in solution but was so far not described in full detail. In this paper we present a complete description of the CYANA implementation of automated NOESY assignment, which differs extensively from its predecessor CANDID by the use of a consistent probabilistic treatment, and we discuss its performance in the second round of the critical assessment of structure determination by NMR.


Automated assignment NOESY Distance restraints Structure calculation CYANA CASD-NMR 



We gratefully acknowledge financial support by the Lichtenberg program of the Volkswagen Foundation and a Grant-in-Aid for Scientific Research of the Japan Society for the Promotion of Science (JSPS).


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Copyright information

© Springer Science+Business Media Dordrecht 2015

Authors and Affiliations

  1. 1.Center for Biomolecular Magnetic Resonance, Institute of Biophysical ChemistryGoethe University Frankfurt am MainFrankfurt am MainGermany
  2. 2.Laboratory of Physical ChemistryETH ZürichZurichSwitzerland
  3. 3.Graduate School of ScienceTokyo Metropolitan UniversityHachiojiJapan

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