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Journal of Biomolecular NMR

, Volume 61, Issue 1, pp 83–87 | Cite as

NMR structure determination of the protein NP_344798.1 as the first representative of Pfam PF06042

  • Biswaranjan Mohanty
  • Pedro Serrano
  • Michael Geralt
  • Kurt Wüthrich
NMR structure note

Biological context

The protein NP_344798.1 from Streptococcuspneumoniae (TIGR4) belongs to the Pfam protein family PF06042, which currently contains 786 sequences from 739 species (http://pfam.xfam.org/family/PF06042). Based on sequence analyses, NP_344798.1 has been identified as a member of the nucleotidyltransfererase (NTase)-fold superfamily and annotated as a protein domain of unknown function, DUF925 (Kuchta et al. 2009). The bioinformatics data further suggest that all PF06042 members contain a single domain and are active NTases, as they contain the characteristic functional catalytic residues (Kuchta et al. 2009). Nonetheless, due to scarcity of experimental data, no specific biological role of these enzymes could as yet be ascertained. The NMR core of the Joint Center for Structural Genomics (JCSG) targeted NP_344798.1 to obtain a first structure for the PF06042 family, and the J-UNIO protocol for automated NMR structure determination (Serrano et al. 2012) was applied to...

Keywords

Complex Point Backbone Assignment Polypeptide Segment Erroneous Assignment Torsion Angle Dynamic 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

This work was funded by the Joint Center for Structural Genomics (JCSG) through the NIH Protein Structure Initiative (PSI) Grant Number U54 GM094586 from the National Institute of General Medical Sciences (www.nigms.nih.gov). BM received support from the Skaggs Institute of Chemical Biology. Kurt Wüthrich is the Cecil H. and Ida M. Green Professor of Structural Biology at TSRI. BM thanks Dr. Reto Horst for assistance in optimizing the setup of NMR experiments.

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Copyright information

© Springer Science+Business Media Dordrecht 2014

Authors and Affiliations

  • Biswaranjan Mohanty
    • 1
    • 2
    • 3
    • 4
  • Pedro Serrano
    • 1
    • 2
  • Michael Geralt
    • 1
    • 2
  • Kurt Wüthrich
    • 1
    • 2
    • 3
  1. 1.Department of Integrative Structural and Computational BiologyThe Scripps Research InstituteLa JollaUSA
  2. 2.Joint Center for Structural GenomicsLa JollaUSA
  3. 3.Skaggs Institute for Chemical BiologyThe Scripps Research InstituteLa JollaUSA
  4. 4.Medicinal Chemistry, Monash Institute of Pharmaceutical SciencesMonash UniversityParkvilleAustralia

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