Journal of Biomolecular NMR

, Volume 58, Issue 3, pp 149–154 | Cite as

A decadentate Gd(III)-coordinating paramagnetic cosolvent for protein relaxation enhancement measurement

Communication

Abstract

Solvent paramagnetic relaxation enhancement (sPRE) arises from random collisions between paramagnetic cosolvent and protein of interest. The sPRE can be readily measured, affording protein structure information. However, lack of an inert cosolvent probe may yield sPRE values that are not consistent with protein structure. Here we synthesized a new sPRE probe, triethylenetetraamine hexaacetate trimethylamide gadolinium, or Gd(III)–TTHA–TMA. With a total of 10 coordination sites, this paramagnetic cosovlent eliminates an inner-sphere water molecule that can otherwise transfer relaxation to protein through exchange. With the metal ion centered, the new probe is largely spherical with a radius of 4.0 Å, permitting accurate back calculation of sPRE. The effectiveness Gd(III)–TTHA–TMA as a sPRE probe was demonstrated on three well-studied protein systems.

Keywords

NMR spectroscopy Protein structures Paramagnetic relaxation enhancement Paramagnetic probe Solvent PRE 

Notes

Acknowledgments

The work was supported by funds from the Ministry of Science and Technology of China (2013CB910200), the National Natural Sciences Foundation of China (21073230, 31170728 and 31125007), and Zhejiang Provincial Natural Science Foundation of China (Z2110059). C.T. is an international early career scientist of Howard Hughes Medical Institute (HHMI).

Supplementary material

10858_2014_9817_MOESM1_ESM.docx (896 kb)
Supplementary material 1 (DOCX 897 kb)

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Copyright information

© Springer Science+Business Media Dordrecht 2014

Authors and Affiliations

  1. 1.State Key Laboratory of Magnetic Resonance and Atomic Molecular Physics and Wuhan Institute of Physics and MathematicsChinese Academy of SciencesWuhanChina
  2. 2.Department of PharmacologyZhejiang University School of MedicineHangzhouChina

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