Journal of Biomolecular NMR

, Volume 57, Issue 2, pp 155–166

Practical considerations over spectral quality in solid state NMR spectroscopy of soluble proteins

  • Marco Fragai
  • Claudio Luchinat
  • Giacomo Parigi
  • Enrico Ravera
Article

DOI: 10.1007/s10858-013-9776-0

Cite this article as:
Fragai, M., Luchinat, C., Parigi, G. et al. J Biomol NMR (2013) 57: 155. doi:10.1007/s10858-013-9776-0

Abstract

Great theoretical and methodological advances are pushing the limits of resolution and sensitivity in solid state NMR (SSNMR). However, sample preparation remains a critical issue for the success of an experiment. The factors affecting spectral quality in SSNMR samples are discussed, examining cases encountered in the literature and presenting new experimental data. A discussion on resolution and sensitivity in sedimented solutes is framed in this context.

Keywords

Ultracentrifuge Sedimentation Solid state NMR Sample preparation Frozen solution Cryoprotection Ubiquitin Superoxide dismutase 

Supplementary material

10858_2013_9776_MOESM1_ESM.doc (72 kb)
Supplementary material 1 (DOC 72 kb)

Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  • Marco Fragai
    • 1
    • 2
  • Claudio Luchinat
    • 1
    • 2
  • Giacomo Parigi
    • 1
    • 2
  • Enrico Ravera
    • 1
    • 2
  1. 1.Center for Magnetic Resonance (CERM)University of FlorenceSesto FiorentinoItaly
  2. 2.Department of ChemistryUniversity of FlorenceSesto FiorentinoItaly

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