PHD domain from human SHPRH
SHPRH (SNF2, histone linker, PHD, RING, helicase) is a 193 kDa member of SWI2/SNF2 family of ATP-dependent chromatin remodeling enzymes (Flaus et al. 2006), containing the C3HC4 (Cys3-His-Cys4) RING-finger characteristic of E3 ubiquitin ligases. The human SHPRH gene maps to the chromosomal region 6q24 whose allelic loss or deletions are observed in many types of cancer, leading to the hypothesis that this region may encode putative tumor suppressor gene(s) (Sood et al. 2003). Consistent with this notion, SHPRH has been subsequently shown to play a key role in the error-free branch of DNA damage tolerance—a mechanism by which cells can bypass replication blocks caused by sites of DNA damage (lesions) and/or fill postreplicational lesion-containing single-stranded DNA gaps (Chang and Cimprich 2009; Unk et al. 2010).
SHPRH, and the closely-related protein, helicase like transcription factor (HLTF), are functional homologues of S. cerevisiaeRad5, acting as ubiquitin...
KeywordsProliferate Cell Nuclear Antigen Insert Region Plant Homeodomain Finger Aromatic Cage Human Proliferate Cell Nuclear Antigen
This work was supported by UCHC startup funds and Agilent Technologies Foundation Grant #2730 to D.M.K., grants from FAPERJ, INCT-INBEB and Science without Boarders (Ciências sem Fronteiras) CNPq program to F.C.L.A., NIH Grant ES016486 to K.A.C., and CAPES sandwich Ph.D. fellowship to L.E.F.M. A.C.K. was funded by NIH training Grant T32CA09151 and American Cancer Society fellowship PF-12-156-01-DMC.
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