Journal of Biomolecular NMR

, Volume 56, Issue 3, pp 191–202 | Cite as

PINT: a software for integration of peak volumes and extraction of relaxation rates

  • Alexandra Ahlner
  • Mats Carlsson
  • Bengt-Harald Jonsson
  • Patrik Lundström


We present the software Peak INTegration (PINT), designed to perform integration of peaks in NMR spectra. The program is very simple to run, yet powerful enough to handle complicated spectra. Peaks are integrated by fitting predefined line shapes to experimental data and the fitting can be customized to deal with, for instance, heavily overlapped peaks. The results can be inspected visually, which facilitates systematic optimization of the line shape fitting. Finally, integrated peak volumes can be used to extract parameters such as relaxation rates and information about low populated states. The utility of PINT is demonstrated by applications to the 59 residue SH3 domain of the yeast protein Abp1p and the 289 residue kinase domain of murine EphB2.


Peak integration Overlapped peaks Relaxation rates Protein dynamics 



We thank Drs. Cecilia Andresen and Janosch Hennig for their help in testing the program and Annica Theresia Blissing for critical reading of the manuscript. The Swedish NMR Center is acknowledged for kindly allowing us to use their spectrometers. This work was supported by a Grant from the Swedish Research Council to P.L.

Supplementary material

10858_2013_9737_MOESM1_ESM.docx (2.7 mb)
Supplementary material 1 (DOCX 2796 kb)


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Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  • Alexandra Ahlner
    • 1
  • Mats Carlsson
    • 1
  • Bengt-Harald Jonsson
    • 1
  • Patrik Lundström
    • 1
  1. 1. Division of Molecular Biotechnology, Department of Physics, Chemistry and BiologyLinköping UniversityLinköpingSweden

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