NMR structure note: solution structure of human Miz-1 zinc fingers 8 to 10
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The transcription factor Miz-1 (Myc-interacting zinc finger protein 1) is a member of the BTB/POZ protein family that contains around 40 human transcription factors. All these proteins bear an evolutionary conserved BTB protein–protein interaction domain at their N-terminus followed by a series of consecutive zinc finger motifs (ZF). Important roles in cell cycle regulation, development and cancer have been attributed to many BTB/POZ proteins demonstrating the relevance to understand the structural biology of those transcription factors (Kelly and Daniel 2006).
Miz-1 was first identified as a direct interactor of the oncogenic protein c-Myc by yeast two-hybrid (Peukert et al. 1997). Miz-1 plays a dual role on the expression of important cell cycle regulator genes such as the cyclin-dependent kinase inhibitors p15INK4 (p15) and p21CIP1(p21) as it can either activate or repress them. Miz-1 acts as an activator of these cytostatic genes by recruiting the machinery...
KeywordsZinc Finger Core Promoter Resonance Assignment TCEP Root Mean Square Deviation
This work was supported by the Natural Science and Engineering Research Council (NSERC) of Canada (grant to P.L. and studentships to M.B. and D.B.) and by the Regroupement stratégique sur la fonction, la structure et l’ingénierie des protéines (PROTEO). D.B. also acknowledges PROTEO for the award of graduate studentship. The authors thank Dr. Frank Hänel (Hans-Knöll-Institut für Naturstoff-Forschung e.V, Germany) for kindly providing us with the Miz-1 cDNA, Dr. Yves L. Dory (Université de Sherbrooke, Canada) for giving access and advice to use his HPLC system, Dr. Jean-François Naud for the initial cloning and Dr. Martin Montagne for his helpful comments on the manuscript. Finally, we wish to thank Prof. Jim Omichinski (U. de Montréal) for his help with the refolding protocol of Zinc Fingers.
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