Simultaneous acquisition of PAR and PAIN spectra
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We present a scheme that allows the simultaneous detection of PAR and PAIN correlation spectra in a single two-dimensional experiment. For both spectra, we obtain almost the same signal-to-noise ratio as if a PAR or PAIN spectrum is recorded separately, which in turn implies that one of the spectra may be considered additional information for free. The experiment is based on the observation that in a PAIN experiment, the PAR condition is always also fulfilled. The performance is demonstrated experimentally using uniformly 13C,15N-labeled samples of N–f–MLF–OH and ubiquitin.
KeywordsSolid-state NMR PAR PAIN CP-MAS
This work was supported by the Swiss National Science Foundation (Grant 200020_134681).The European Commission under the Seventh Framework Program (FP7), contract Bio-NMR 261863 and the Danish National Research Foundation.
- De Paepe G, Lewandowski JR, Loquet A, Eddy M, Megy S, Böckmann A, Griffin RG (2011) Heteronuclear proton assisted recoupling. J Chem Phys 134(9):095101–095119Google Scholar
- Gath J, Habenstein B, Bousset L, Melki R, Meier BH, Böckmann A (2011) Solid-state NMR sequential assignments of α-synuclein. Biomol NMR Assign. doi: 10.1007/s12104-011-9324-3