Journal of Biomolecular NMR

, Volume 50, Issue 2, pp 147–155 | Cite as

relaxGUI: a new software for fast and simple NMR relaxation data analysis and calculation of ps-ns and μs motion of proteins

  • Michael Bieri
  • Edward J. d’Auvergne
  • Paul R. GooleyEmail author


Investigation of protein dynamics on the ps-ns and μs-ms timeframes provides detailed insight into the mechanisms of enzymes and the binding properties of proteins. Nuclear magnetic resonance (NMR) is an excellent tool for studying protein dynamics at atomic resolution. Analysis of relaxation data using model-free analysis can be a tedious and time consuming process, which requires good knowledge of scripting procedures. The software relaxGUI was developed for fast and simple model-free analysis and is fully integrated into the software package relax. It is written in Python and uses wxPython to build the graphical user interface (GUI) for maximum performance and multi-platform use. This software allows the analysis of NMR relaxation data with ease and the generation of publication quality graphs as well as color coded images of molecular structures. The interface is designed for simple data analysis and management. The software was tested and validated against the command line version of relax.


Graphical user interface GUI Lipari and Szabo model-free analysis NMR spin relaxation Protein dynamics Relax software 



Chemical shift anisotropy


Graphical user interface


Heteronuclear single quantum coherence


Olfactory marker protein


Nuclear overhauser effect


Chemical exchange relaxation



This work was supported by equipment grants from the Australian Research Council (ARC) and the Rowden White Foundation. M. B. is a recipient of a Swiss National Science Foundation (SNF) fellowship. We would like to thank Sébastien Morin for supplying the PSE-4 and TEM-1 data.

Supplementary material

10858_2011_9509_MOESM1_ESM.pdf (2 mb)
Supplementary material 1 (PDF 2033 kb)


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Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • Michael Bieri
    • 1
  • Edward J. d’Auvergne
    • 2
  • Paul R. Gooley
    • 1
    Email author
  1. 1.Department of Biochemistry and Molecular BiologyBio21 Molecular Science and Biotechnology Institute, The University of MelbourneParkvilleAustralia
  2. 2.Department of NMR-based Structural BiologyMax Planck Institute for Biophysical ChemistryGoettingenGermany

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