Journal of Biomolecular NMR

, Volume 47, Issue 4, pp 283–288 | Cite as

NMR structure note: solution structure of the core domain of MESD that is essential for proper folding of LRP5/6

  • Jianglei Chen
  • Qianqian Li
  • Chia-Chen Liu
  • Bei Zhou
  • Guojun Bu
  • Jianjun Wang
NMR Structure Note

Biological context

Low-density lipoprotein receptor (LDLR) family controls diverse developmental and physiological pathways, including an endocytic cargo function and signaling capacities (Herz and Bock 2002). LDLR family members belong to type I transmembrane proteins that contain repeating modules, including the cysteine-rich repeats (LDL-A), making up the ligand-binding domains, a BP domain formed by six YWTD repeats (β-propeller) and an epidermal growth factor (EGF) repeat. The number and arrangement of these modules vary among family members. LRP5/6, a co-receptor for binding to Wnts in the Wnt signaling pathway, start with a BP domain, which repeats four times, followed by three LDL-A repeats. The extracellular side is anchored on the plasma membrane by a single transmembrane segment, followed by a cytoplasmic tail containing NPXY signals for endocytosis and interaction motifs for a variety of cytoplasmic adaptor and scaffolding proteins (Strickland et al. 2002).

An ER-resident...


Chemical Shift Change Flexible Loop Culi W32R Mutation Conserve Lysine Residue 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



This work is supported by NIH RO1 grants (HL074365 and HL076620 to JW). The authors also thank Ms Victoria Murray for critical reading of the manuscript.

Supplementary material

10858_2010_9426_MOESM1_ESM.xls (12 kb)
Supplementary material 1 (XLS 11 kb)


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Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  • Jianglei Chen
    • 1
  • Qianqian Li
    • 1
  • Chia-Chen Liu
    • 2
  • Bei Zhou
    • 3
  • Guojun Bu
    • 2
  • Jianjun Wang
    • 1
  1. 1.Department of Biochemistry and Molecular Biology, School of MedicineWayne State UniversityDetroitUSA
  2. 2.Departments of Pediatrics, and Cell Biology and Physiology, School of MedicineWashington UniversitySt. LouisUSA
  3. 3.Department of BiochemistryDuke University Medical CenterDurhamUSA

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