Journal of Biomolecular NMR

, Volume 47, Issue 2, pp 155–162

Comparison of fast backbone dynamics at amide nitrogen and carbonyl sites in dematin headpiece C-terminal domain and its S74E mutant

Article

DOI: 10.1007/s10858-010-9417-9

Cite this article as:
Vugmeyster, L., Ostrovsky, D. & Li, Y. J Biomol NMR (2010) 47: 155. doi:10.1007/s10858-010-9417-9

Abstract

We perform a detailed comparison of fast backbone dynamics probed at amide nitrogen versus carbonyl carbon sites for dematin headpiece C-terminal domain (DHP) and its S74E mutant (DHPS74E). Carbonyl dynamics is probed via auto-correlated longitudinal rates and transverse C′/C′-Cα CSA/dipolar and C′/C′–N CSA/dipolar cross-correlated rates, while 15N data are taken from a previous study. Resulting values of effective order parameters and internal correlation times support the conclusion that C′ relaxation reports on a different subset of fast motions compared to those probed at N–H bond vectors in the same peptide planes. 13C′ order parameters are on the average 0.08 lower than 15N order parameters with the exception of the flexible loop region in DHP. The reduction of mobility in the loop region upon the S74E mutation can be seen from the 15N order parameters but not from the 13C order parameters. Internal correlation times at 13C′ sites are on the average an order of magnitude longer than those at 15N sites for the well-structured C-terminal subdomains, while the more flexible N-terminal subdomains have more comparable average internal correlation times.

Keywords

Dematin headpiece NMR relaxation Backbone dynamics Carbonyl dynamics Fast motions Model-free 

Supplementary material

10858_2010_9417_MOESM1_ESM.pdf (90 kb)
(PDF 90 kb)

Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  1. 1.Department of Chemistry and Environment and Natural Resources InstituteUniversity of Alaska at AnchorageAnchorageUSA
  2. 2.Department of Mathematical SciencesUniversity of Alaska at AnchorageAnchorageUSA
  3. 3.Department of Biochemistry & Molecular BiophysicsColumbia UniversityNew YorkUSA

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