Journal of Biomolecular NMR

, 45:357 | Cite as

TROSY-selected ZZ-exchange experiment for characterizing slow chemical exchange in large proteins



A TROSY-selected ZZ-exchange experiment is described for measuring slow chemical exchange rates by monitoring the TROSY component of 15N longitudinal magnetization. Application of the proposed pulse sequence to the cadherin 8 N-terminal extracelluar domain demonstrates that enhanced sensitivity is obtained, compared to a previously described TROSY-detected ZZ-exchange sequence (Sahu et al. J Am Chem Soc 129: 13232–13237, 2007), by preserving the TROSY effect during the mixing period as well as the frequency encoding periods.


ZZ-exchange TROSY Chemical exchange Kinetic rates Cadherin 

Supplementary material

10858_2009_9385_MOESM1_ESM.pdf (431 kb)
Supplementary material (PDF 430 kb)


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Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  1. 1.Department of Biochemistry and Molecular BiophysicsColumbia UniversityNew YorkUSA

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