TROSY-selected ZZ-exchange experiment for characterizing slow chemical exchange in large proteins
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A TROSY-selected ZZ-exchange experiment is described for measuring slow chemical exchange rates by monitoring the TROSY component of 15N longitudinal magnetization. Application of the proposed pulse sequence to the cadherin 8 N-terminal extracelluar domain demonstrates that enhanced sensitivity is obtained, compared to a previously described TROSY-detected ZZ-exchange sequence (Sahu et al. J Am Chem Soc 129: 13232–13237, 2007), by preserving the TROSY effect during the mixing period as well as the frequency encoding periods.
KeyWordsZZ-exchange TROSY Chemical exchange Kinetic rates Cadherin
This work was supported by National Institutes of Health (NIH) grant GM59273 (A. G. P.). We thank Fabiana Bahna and Prof. Lawrence Shapiro for assistance in sample preparation.
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