Journal of Biomolecular NMR

, Volume 45, Issue 1–2, pp 227–232 | Cite as

Characterization of slow conformational dynamics in solids: dipolar CODEX

Article

Abstract

A solid state NMR experiment is introduced for probing relatively slow conformational exchange, based on dephasing and refocusing dipolar couplings. The method is closely related to the previously described Centerband-Only Detection of Exchange or CODEX experiment. The use of dipolar couplings for this application is advantageous because their values are known a priori from molecular structures, and their orientations and reorientations relate in a simple way to molecular geometry and motion. Furthermore the use of dipolar couplings in conjunction with selective isotopic enrichment schemes is consistent with selection for unique sites in complex biopolymers. We used this experiment to probe the correlation time for the motion of 13C, 15N enriched urea molecules within their crystalline lattice.

Keywords

Dynamics Solid-state NMR CODEX Chemical exchange Magic angle spinning Urea 

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Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  1. 1.Department of ChemistryColumbia UniversityNew YorkUSA

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