Solution structure of GSP13 from Bacillus subtilis exhibits an S1 domain related to cold shock proteins
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GSP13 encoded by gene yugI is a σB-dependent general stress protein in Bacillus subtilis, which can be induced by heat shock, salt stress, ethanol stress, glucose starvation, oxidative stress and cold shock. Here we report the solution structure of GSP13 and it is the first structure of S1 domain containing protein in Bacillus subtilis. The structure of GSP13 mainly consists of a typical S1 domain along with a C-terminal 50-residue flexible tail, different from the other known S1 domain containing proteins. Comparison with other S1 domain structures reveals that GSP13 has a conserved RNA binding surface, and it may function similarly to cold shock proteins in response to cold stress.
KeywordsGSP GSP13 yugI S1 domain Cold shock protein
All NMR experiments were carried out at the Beijing NMR Center. We thank Dr. Xiao-Dong Su for providing us the genome library of Bacillus subtilis. This research was supported by Grant 30125009 to BX from the National Science Foundation of China and grant 2006AA02A323 to CJ from the 863 Program.
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