Journal of Biomolecular NMR

, Volume 43, Issue 1, pp 51–61 | Cite as

Design of high-power, broadband 180° pulses and mixing sequences for fast MAS solid state chemical shift correlation NMR spectroscopy

  • Christian Herbst
  • Jirada Herbst
  • Anika Kirschstein
  • Jörg Leppert
  • Oliver Ohlenschläger
  • Matthias Görlach
  • Ramadurai Ramachandran
Article

Abstract

An approach for the design of high-power, broadband 180° pulses and mixing sequences for generating dipolar and scalar coupling mediated 13C–13C chemical shift correlation spectra of isotopically labelled biological systems at fast magic-angle spinning frequencies without 1H decoupling during mixing is presented. Considering RF field strengths in the range of 100–120 kHz, as typically available in MAS probes employed at high spinning speeds, and limited B1 field inhomogeneities, the Fourier coefficients defining the phase modulation profile of the RF pulses were optimised numerically to obtain broadband inversion and refocussing pulses and mixing sequences. Experimental measurements were carried out to assess the performance characteristics of the mixing sequences reported here.

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Copyright information

© Springer Science+Business Media B.V. 2008

Authors and Affiliations

  • Christian Herbst
    • 1
  • Jirada Herbst
    • 2
  • Anika Kirschstein
    • 1
  • Jörg Leppert
    • 1
  • Oliver Ohlenschläger
    • 1
  • Matthias Görlach
    • 1
  • Ramadurai Ramachandran
    • 1
  1. 1.Research group Biomolecular NMR spectroscopyLeibniz Institute for Age Research, Fritz Lipmann InstituteJenaGermany
  2. 2.Department of Mathematics, Statistics and Computer, Faculty of ScienceUbon Ratchathani UniversityUbon RatchathaniThailand

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