Journal of Biomolecular NMR

, Volume 42, Issue 3, pp 155–158 | Cite as

Solution NMR structure determination of proteins revisited

Perspective

Abstract

This ‘Perspective’ bears on the present state of protein structure determination by NMR in solution. The focus is on a comparison of the infrastructure available for NMR structure determination when compared to protein crystal structure determination by X-ray diffraction. The main conclusion emerges that the unique potential of NMR to generate high resolution data also on dynamics, interactions and conformational equilibria has contributed to a lack of standard procedures for structure determination which would be readily amenable to improved efficiency by automation. To spark renewed discussion on the topic of NMR structure determination of proteins, procedural steps with high potential for improvement are identified.

Keywords

NMR structure determination of proteins Protein biochemistry Automation Structural biology Structural genomics 

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Copyright information

© Springer Science+Business Media B.V. 2008

Authors and Affiliations

  • Martin Billeter
    • 1
  • Gerhard Wagner
    • 2
  • Kurt Wüthrich
    • 3
    • 4
  1. 1.Department of ChemistryUniversity of GothenburgGothenburgSweden
  2. 2.Department of Biological Chemistry and Molecular PharmacologyHarvard Medical SchoolBostonUSA
  3. 3.Department of Molecular Biology and The Skaggs Institute for Chemical BiologyThe Scripps Research InstituteLa JollaUSA
  4. 4.Institute of Molecular Biology and BiophysicsETH ZürichZurichSwitzerland

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