Joint composite-rotation adiabatic-sweep isotope filtration
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Joint composite-rotation adiabatic-sweep isotope filters are derived by combining the composite-rotation [Stuart AC et al. (1999) J Am Chem Soc 121: 5346–5347] and adiabatic-sweep [Zwahlen C et al. (1997) J Am Chem Soc 119:6711–6721; Kupče E, Freeman R (1997) J Magn Reson 127:36–48] approaches. The joint isotope filters have improved broadband filtration performance, even for extreme values of the one-bond 1H–13C scalar coupling constants in proteins and RNA molecules. An average Hamiltonian analysis is used to describe evolution of the heteronuclear scalar coupling interaction during the adiabatic sweeps within the isotope filter sequences. The new isotope filter elements permit improved selective detection of NMR resonance signals originating from 1H spins attached to an unlabeled natural abundance component of a complex in which the other components are labeled with 13C and 15N isotopes.
KeywordsComposite pulse J-filter NOESY Structure determination vts SAM domain
This work was supported by NIH grants GM50291 (A.G. P.) and GM47021 (D. C.). E.R.V. acknowledges support from a National Science Foundation Graduate Research Fellowship. We thank Joel A. Butterwick (Columbia University), Thomas A. Edwards (Mt. Sinai School of Medicine), and Aneel K. Aggarwal (Mt. Sinai School of Medicine) for the Vts1 SAM domain/TCE 13mer RNA sample. Helpful discussions with Mark Rance (Univ. Cincinnati) are acknowledged gratefully.
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