Random sampling of evolution time space and Fourier transform processing
Application of Fourier Transform for processing 3D NMR spectra with random sampling of evolution time space is presented. The 2D FT is calculated for pairs of frequencies, instead of conventional sequence of one-dimensional transforms. Signal to noise ratios and linewidths for different random distributions were investigated by simulations and experiments. The experimental examples include 3D HNCA, HNCACB and 15N-edited NOESY-HSQC spectra of 13C 15N labeled ubiquitin sample. Obtained results revealed general applicability of proposed method and the significant improvement of resolution in comparison with conventional spectra recorded in the same time.
Key wordsfast multidimensional NMR Fourier Transform random sampling ubiquitin
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Authors are grateful to Prof. Andrew R. Byrd (Structural Biophysics Laboratory, National Cancer Institute-Frederick, Frederick, Maryland, USA) for the sample of 13C, 15N-double labeled human ubiquitin.
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