Journal of Biomolecular NMR

, Volume 35, Issue 2, pp 149–154

MTH187 from Methanobacterium thermoautotrophicum has three HEAT-like Repeats

  • Olivier Julien
  • Isabelle Gignac
  • Anna Hutton
  • Adelinda Yee
  • Cheryl H. Arrowsmith
  • Stéphane M. Gagné
NMR Structure Note

Abstract

With the completion of genome sequencing projects, there are a large number of proteins for which we have little or no functional information. Since protein function is closely related to three-dimensional conformation, structural proteomics is one avenue where the role of proteins with unknown function can be investigated. In the present structural project, the structure of MTH187 has been determined by solution-state NMR spectroscopy. This protein of 12.4 kDa is one of the 424 non-membrane proteins that were cloned and purified for the structural proteomic project of Methanobacterium thermoautotrophicum [Christendat, D., Yee, A., Dharamsi, A., Kluger, Y., Gerstein, M., Arrowsmith, C.H. and Edwards, A.M. (2000) Prog. Biophys. Mol. Biol., 73, 339–345]. Methanobacterium thermoautotrophicum is a thermophilic archaeon that grows optimally at 65 °C. A particular characteristic of this microorganism is its ability to generate methane from carbon dioxide and hydrogen [Smith, D.R., Doucette-Stamm, L.A., Deloughery, C., Lee, H., Dubois, J., Aldredge, T., Bashirzadeh, R., Blakely, D., Cook, R., Gilbert, K., Harrison, D., Hoang, L., Keagle, P., Lumm, W., Pothier, B., Qiu, D., Spadafora, R., Vicaire, R., Wang, Y., Wierzbowski, J., Gibson, R., Jiwani, N., Caruso, A., Bush, D., Reeve, J. N. et al. (1997) J. Bacteriol., 179, 7135–7155].

Keywords

HEAT-like repeat Methanobacterium thermoautotrophicum MTH187 NMR protein structure structural proteomics 

Abbreviations

DSS

2,2-dimethyl-silapentane-5-sulfonic acid

NOE

nuclear Overhauser effect

NOESY

NOE spectroscopy

PSI-BLAST

Position-Specific Iterative Basic Local Alignment Search Tool

RMSD

root-mean-square deviation

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Supplementary material

10858_2006_29_MO1_ESM.pdf (1010 kb)
Supplementary material

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Copyright information

© Springer 2006

Authors and Affiliations

  • Olivier Julien
    • 1
  • Isabelle Gignac
    • 1
  • Anna Hutton
    • 2
  • Adelinda Yee
    • 3
  • Cheryl H. Arrowsmith
    • 3
  • Stéphane M. Gagné
    • 1
  1. 1.Département de biochimie et de microbiologie and CREFSIPUniversité LavalQuébecCanada
  2. 2.Institute for Biomolecular DesignUniversity of AlbertaEdmontonCanada
  3. 3.Division of Molecular and Structural Biology, Department of Medical Biophysics, Ontario Cancer InstituteUniversity of TorontoTorontoCanada

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