Journal of Biomolecular NMR

, Volume 34, Issue 2, pp 101–115 | Cite as

A Thorough Dynamic Interpretation of Residual Dipolar Couplings in Ubiquitin

  • Nils A. Lakomek
  • Teresa Carlomagno
  • Stefan Becker
  • Christian Griesinger
  • Jens Meiler


The presence of slow motions with large amplitudes, as detected by measurements based on residual dipolar couplings [Peti, W., Meiler, J., Brueschweiler, R. and Griesinger, C. (2002) J. Am. Chem. Soc., 124, 5822–5833], has stirred up much discussion in recent years. Based on ubiquitin NH residual dipolar couplings (rdcs) measured in 31 different alignment conditions, a model-free analysis of structure and dynamics [Meiler, J., Peti, W., Prompers, J., Griesinger, C. and Brueschweiler, R. (2001) J. Am. Chem. Soc., 123, 6098–6107] is presented. Starting from this broad experimental basis, rdc-based order parameters with so far unattained accuracy were determined. These rdc-based order parameters underpin the presence of new modes of motion slower than the inverse overall tumbling correlation time. Amplitudes and anisotropies of the motion were derived. The effect of structural noise on the results was proven to be negligible.


dynamical averaging model free approach nuclear magnetic resonance (NMR) residual dipolar couplings (rdcs) ubiquitin 


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Copyright information

© Springer 2006

Authors and Affiliations

  • Nils A. Lakomek
    • 1
  • Teresa Carlomagno
    • 1
  • Stefan Becker
    • 1
  • Christian Griesinger
    • 1
  • Jens Meiler
    • 2
  1. 1.NMR based structural biologyMax Planck Institute for Biophysical ChemistryGoettingenGermany
  2. 2.Center of Structural Biology, Department of ChemistryVanderbilt UniversityNashvilleUSA

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