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Combinational delivery of anticancer drugs for osteosarcoma treatment using electrosprayed core shell nanocarriers

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Abstract

In bone cancer treatment, local delivery of chemotherapeutic agents is preferred compared to other routes of administration. Delivery of multiple drugs using biodegradable carriers improves the treatment efficiency and overcomes drug resistance and toxicity. With this approach, we have developed multilayer biodegradable core shell nanoparticles (NPs) using the electro-spraying technique to deliver methotrexate (MTX) and doxorubicin (DOX) for the treatment of osteosarcoma. These core-shell NPs with a mean particle size of 212 ± 41 nm consist of hydroxyapatite (HA) and DOX as core with the outer shell made of chitosan (CH) followed by polycaprolactone (PCL) with MTX. The encapsulation efficiency of MTX was around 85% and DOX was 38%. In vitro drug release studies were performed in phosphate buffered saline (PBS) at pH 5 and pH 7.4 for 8 days. Different release profiles were observed in both acidic and alkaline pH. The sequential release of MTX followed by DOX was observed in both pH in sustained manner. Human osteosarcoma MG 63 (OMG-63) cells lines were used to test the cytotoxicity of drug loaded NPs. Multi-drug encapsulated bioresorbable and biodegradable electro-sprayed core shell NPs will be promising as a bone substitute for the treatment of osteosarcoma.

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Acknowledgements

The authors thank Dr. Anant Raheja and Dr. Rajashekhar Bijja, FIB SOL Life technologies, Chennai for electrospraying instrument and their support and guidance in electrospraying optimization. The authors thank Prof. Nitish R Mahapatra and Prof. Suresh Kumar Rayala for providing cell culture facility and Prof. Sakthi Kumar of Toyo University, Japan for the TEM. The authors also thank Sophisticated Analytical Instrument Facility (SAIF) and Central XRD Facility of IIT Madras for analytical support.

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  1. A. Jayakrishnan

    Present address: Raja Ramanna Fellow, Rajiv Gandhi Centre for Biotechnology, Jagathy, Trivandrum, 695 014, Kerala, India

Authors and Affiliations

  1. Biomaterials Laboratory, Department of Biotechnology, Bhupat and Jyoti Mehta School of Biosciences, Indian Institute of Technology Madras, Chennai, India

    S. Ram Prasad & A. Jayakrishnan

  2. Medical Materials Laboratory, Department of Metallurgical and Materials Engineering, Indian Institute of Technology Madras, Chennai, 600 036, Tamil Nadu, India

    S. Ram Prasad & T. S. Sampath Kumar

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Prasad, S.R., Jayakrishnan, A. & Kumar, T.S.S. Combinational delivery of anticancer drugs for osteosarcoma treatment using electrosprayed core shell nanocarriers. J Mater Sci: Mater Med 31, 44 (2020). https://doi.org/10.1007/s10856-020-06379-5

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