Stretchable collagen-coated polyurethane-urea hydrogel seeded with bladder smooth muscle cells for urethral defect repair in a rabbit model
- 5 Downloads
The major challenge to treat the clinical adverse effects of long-segment urethra is in achieving viable tissue substitution. The substituted construct’s properties-such as its resilience, contraction, and ability to minimize scar-stenosis formation should be considered. In the present work, a unique polyurethane-urea (PUU) fibrous membrane is fabricated by electrospinning. Then PUU was coated by collagen and formed the elasticity hydrogel after immersed in collagen solution. Meanwhile, the cPUU hydrogel exhibited a fibrous microstructure. This cPUU hydrogel had outstanding stretching property with 404 ± 40% elongation at break compared with traditional hydrogels, which satisfied the requirement of urethra. The cPUU hydrogel also supported the adhesion and growth of bladder smooth-muscle cells (BSMCs) in natural state cell morphology. Urethral defects in New Zealand male rabbits were repaired with cPUU seeded with BSMCs in vivo. After three months, more smooth-surface area of reconstructed urethral tissues was observed in the cPUU hydrogel-BMSCs groups compared with that of the control group. The luminal patency and the incidence of complications-including calculus formation, urinary fistula, and urethral-stricture occurrence were significantly lower in the cPUU group compared with that of the control group. Hence, cPUU fibrous hydrogels are promising scaffolds for application in urological tissue engineering.
This study was supported by the National Key R&D Program of China (2016YFC1100203), National Natural Science Foundation of China (81672213, 31872748), Jiangsu Provincial Clinical Orthopedic Center, and the Priority Academic Program Development (PAPD) of Jiangsu Higher Education Institutions.
Weiguo Chen, Fengxuan Han and Bin Li conceived and designed the experiments; Professor Mingyu Guo provided the PUU materials; Chunyang Chen and Chengyuan Wang performed the experiments with the help of Fengxuan Han; Chunyang Chen and Chengyuan Wang analyzed the data; Chengyuan Wang and Fengxuan Han wrote the paper.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
- 17.El-Sherbiny IM, Yacoub MH. Hydrogel scaffolds for tissue engineering: progress and challenges. Glob Cardiol Sci Pr. 2013;2013:316–42.Google Scholar
- 18.Stier S, Rebers L, Schönhaar V, Hoch E, Borchers K. Advanced formulation of methacryl- and acetyl-modified biomolecules to achieve independent control of swelling and stiffness in printable hydrogels. J Mater Sci. 2019;30:35.Google Scholar
- 20.Guo T, Yang X, Deng J, Zhu L, Wang B, Hao S. Keratin nanoparticles-coating electrospun PVA nanofibers for potential neural tissue applications. J Mater Sci. 2018;30:9.Google Scholar
- 23.Yang B-Y, Deng G-Y, Zhao R-Z, Dai C-Y, Jiang C-Y, Wang X-J, et al. Porous Se@SiO2 nanosphere-coated catheter accelerates prostatic urethra wound healing by modulating macrophage polarization through reactive oxygen species-NF-κB pathway inhibition. Acta Biomaterialia. 2019;88:392–405.CrossRefGoogle Scholar
- 34.Shakhssalim N, Soleimani M, Dehghan MM, Rasouli J, Taghizadeh-Jahed M, Torbati PM, et al. Bladder smooth muscle cells on electrospun poly(epsilon-caprolactone)/poly(l-lactic acid) scaffold promote bladder regeneration in a canine model. Mater Sci Eng C Mater Biol Appl. 2017;75:877–84.CrossRefGoogle Scholar