14-3-3ε protein-immobilized PCL-HA electrospun scaffolds with enhanced osteogenicity

  • G. Rivero
  • A. A. Aldana
  • Y. R. Frontini Lopez
  • L. Liverani
  • A. R. Boccacini
  • D. M. Bustos
  • G. A. AbrahamEmail author
Tissue Engineering Constructs and Cell Substrates Rapid Communication
Part of the following topical collections:
  1. Tissue Engineering Constructs and Cell Substrates


Adipose-derived mesenchymal stem cells (ASCs) accelerate the osteointegration of bone grafts and improve the efficiency in the formation of uniform bone tissue, providing a practical and clinically attractive approach in bone tissue regeneration. In this work, the effect of nanofibrous biomimetic matrices composed of poly(ε-caprolactone) (PCL), nanometric hydroxyapatite (nHA) particles and 14-3-3 protein isoform epsilon on the initial stages of human ASCs (hASCs) osteogenic differentiation was investigated. The cells were characterized by flow cytometry and induction to differentiation to adipogenic and osteogenic lineages. The isolated hASCs were induced to differentiate to osteoblasts over all scaffolds, and adhesion and viability of the hASCs were found to be similar. However, the activity of alkaline phosphatase (ALP) as early osteogenic marker in the PCL-nHA/protein scaffold was four times higher than in PCL-nHA and more than five times than the measured in neat PCL.



The authors thank the MINCyT-DAAD 2016 binational cooperation project for partial funding.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.


  1. 1.
    Kenry LCT. Nanofiber technology: current status and emerging developments. Progr Polym Sci. 2017;70:1–17.CrossRefGoogle Scholar
  2. 2.
    Rivero G, Aldana AA, Abraham GA. Nanocomposite electrospun micro/nanofibers for biomedical applications. In: Grumezescu V, Grumezescu AM, editors. Materials for biomedical engineering: biopolymer Fibers. Amsterdam: Elsevier; 2019. pp. 89–126.Google Scholar
  3. 3.
    He C, Nie W, Feng W. Engineering of biomimetic nanofibrous matrices of drug delivery and tissue engineering. J Mater Chem B. 2014;2:7828–48.CrossRefGoogle Scholar
  4. 4.
    Dang M, Saunders L, Niu X, Fan Y, Ma PX. Biomimetic delivery of signals for bone tissue engineering. Bone Res. 2018;6:25.CrossRefGoogle Scholar
  5. 5.
    Zuk PA, Zhu M, Mizuno H, Futrell JW, Katz AJ, Benhaim P. et al. Multilineage cells from human adipose tissue: implications for cell-based therapies. Tissue Eng. 2001;7:211–28.CrossRefGoogle Scholar
  6. 6.
    Romagnoli C, Brandi ML. Adipose mesenchymal stem cells in the field of bone tissue engineering. World J Stem Cells. 2014;6:144–52.CrossRefGoogle Scholar
  7. 7.
    Thomas D, Guthridge M, Woodcock J, Lopez A. 14-3-3 protein signaling in development and growth factor responses. Curr Top Dev Biol. 2005;67:285–303.CrossRefGoogle Scholar
  8. 8.
    Kleppe R, Martinez A, Døskeland S, Haavik J. The 14-3-3 proteins in regulation of cellular metabolism. Semin Cell Dev Biol. 2011;22:713–9.CrossRefGoogle Scholar
  9. 9.
    Frontini-Lopez YR, Gojanovich AD, Masone D, Bustos D, Uhart M. Adipose-derived mesenchymal stem/stromal cells: from the lab bench to the basic concepts for clinical translation. BIOCELL. 2018;42:67–77.Google Scholar
  10. 10.
    Nefla M, Sudre L, Denat G, Priam S, Andre-Leroux G, Berenbaum F. et al. The pro-inflammatory cytokine 14-3-3ε is a ligand of CD13 in cartilage. J Cell Sci. 2015;128:3250–62.CrossRefGoogle Scholar
  11. 11.
    Liverani L, Boccaccini AR. Versatile production of poly(epsilon-caprolactone) fibers by electrospinning using benign solvents. Nanomaterials. 2016;6:75.CrossRefGoogle Scholar
  12. 12.
    Uhart M, Iglesias AA, Bustos DM. Structurally constrained residues outside the binding motif are essential in the interaction of 14-3-3 and phosphorylated partner. J Mol Biol. 2011;406:552–7.CrossRefGoogle Scholar
  13. 13.
    Guler Z, Sarac AS. Electrochemical impedance and spectroscopy study of the EDC/NHS activation of the carboxyl groups on poly(ε-caprolactone)/poly(m-anthranilic acid) nanofibers. Express Lett. 2016;10:96–110.CrossRefGoogle Scholar
  14. 14.
    Gojanovich AD, Masone D, Rodriguez TM, Dewey RA, Delgui LR, Bustos DM. et al. Human adipose-derived mesenchymal stem/stromal cells handling protocols. Lipid droplets and proteins double-staining. Front Cell Dev Biol. 2018;6:33.CrossRefGoogle Scholar
  15. 15.
    Miron RJ, Zhang YF. Osteoinduction: a review of old concepts with new standards. J Dent Res. 2012;91:736–44.CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Instituto de Investigaciones en Ciencia y Tecnología de Materiales, INTEMA (UNMdP-CONICET)Mar del PlataArgentina
  2. 2.Institute of Biomaterials, Department of Materials Science and EngineeringUniversity of Erlangen-NurembergErlangenGermany
  3. 3.Laboratorio de Integración de Señales CelularesInstituto de Histología y Embriología de Mendoza (IHEM-CONICET-UNCUYO)MendozaArgentina
  4. 4.Facultad de Ciencias Exactas y Naturales (UNCuyo)MendozaArgentina

Personalised recommendations