Advertisement

The osteoinduction of RGD and Mg ion functionalized bioactive zirconia coating

  • Zhengfei Huang
  • Zhifeng Wang
  • Chuanhua Li
  • Ning Zhou
  • Fei Liu
  • Jing LanEmail author
Biocompatibility Studies Original Research
  • 32 Downloads
Part of the following topical collections:
  1. Biocompatibility Studies

Abstract

The objective of this study was to investigate the adhesion, proliferation and mineralization of osteoblasts on arginine-glycine-aspartic acid (RGD)- and magnesium ion (Mg+)-decorated zirconia coatings. The zirconia coatings were prepared via a plasma spray; RGD and Mg+ were immobilized via a silane-coupling agent and ion implantation, respectively. This study employed scanning electron microscopy (SEM) to observe the surface morphology of RGD- and Mg+-decorated zirconia coatings; surface roughness and wettability were also measured. The initial adhesion of osteoblasts was measured, and cell morphology and focal adhesion were observed. In addition, the expressions of the integrins a1, a2, a5, av, and ß1 were measured using RT-PCR. A cell count was conducted to measure proliferation. The expressions of ALP and OCN were detected based on a western blot analysis, and mineralized nodules were observed to visualize the mineralization of osteoblasts. A nanoscale surface structure could be found on the Mg+-decorated zirconia coating, and the RGD-decorated zirconia coating showed better wettability (p < 0.05). Cells on the RGD- and Mg+-decorated zirconia coating possessed better spreading properties than did cells on nondecorated surfaces, and more focal adhesion was observed. The higher expressions of the integrins a5, av and ß1 were found on the RGD-decorated zirconia coating (p < 0.05). The western blot results demonstrated that the introduction of Mg+ heightened the expressions of ALP and OCN. More and bigger mineralized nodules were observed on the Mg+- and RGD-decorated zirconia coating, which consisted of small mineralized nodules. RGD- and Mg+-functionalized zirconia coating facilitates the osteogenic reaction of osteoblasts. RGD improves the adhesion of osteoblasts, and Mg+ benefits the mineralization of osteoblasts. In addition, a synergistic effect was found between RGD and Mg+, allowing better performances with regard to adhesion, proliferation and mineralization when the two were used together rather than as separate decorations.

Notes

Acknowledgements

The work was funded by grants 81671025 (Beijing, China) from National Natural Science Foundation of China, 2015GSF118186 (Jinan, China) and ZR2018ZB0105 (Ji’nan, China) from Foundation of Department of Science and Technology of Shandong province.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

References

  1. 1.
    Höhn S, Virtanen S. Effect of inflammatory conditions and H2O2 on bare and coated Ti–6Al–4V surfaces: corrosion behavior, metal ion release and Ca-P formation under long-term immersion in DMEM. Appl Surf Sci. 2015;357:101–11.CrossRefGoogle Scholar
  2. 2.
    Ko N, Mine A, Egusa H, Shimazu T, Ko R, Nakano T, et al. Allergic reaction to titanium-made fixed dental restorations: a clinical report. J Prosthodont. 2014;23:501–3.CrossRefGoogle Scholar
  3. 3.
    Cosgarea R, Gasparik C, Dudea D, Culic B, Dannewitz B, Sculean A. Peri-implant soft tissue colour around titanium and zirconia abutments: a prospective randomized controlled clinical study. Clin Oral Implants Res. 2015;26:537–44.CrossRefGoogle Scholar
  4. 4.
    Gahlert M, Burtscher D, Grunert I, Kniha H, Steinhauser E. Failure analysis of fractured dental zirconia implants. Clin Oral Implants Res. 2012;23:287–93.CrossRefGoogle Scholar
  5. 5.
    Osman RB, Ma S, Duncan W, De Silva RK, Siddiqi A, Swain MV. Fractured zirconia implants and related implant designs: scanning electron microscopy analysis. Clin Oral Implants Res. 2013;24:592–7.CrossRefGoogle Scholar
  6. 6.
    Hao L, Lawrence J, Chian KS. Osteoblast cell adhesion on a laser modified zirconia based bioceramic. J Mater Sci Mater Med. 2005;16:719–26.CrossRefGoogle Scholar
  7. 7.
    Sener-Yamaner ID, Yamaner G, Sertgoz A, Canakci CF, Ozcan M. Marginal bone loss around early-loaded SLA and SLActive Implants: radiological follow-up evaluation up to 6.5 years. Implant Dent. 2017;26:592–9.CrossRefGoogle Scholar
  8. 8.
    Shah FA, Johansson ML, Omar O, Simonsson H, Palmquist A, Thomsen P. Laser-modified surface enhances osseointegration and biomechanical anchorage of commercially pure titanium implants for bone-anchored hearing systems. PLoS ONE. 2016;11:e0157504.CrossRefGoogle Scholar
  9. 9.
    Zahran R, Rosales LJ, Rodriguez VM, Cabrerizo VM. Effect of hydrofluoric acid etching time on titanium topography, chemistry, wettability, and cell adhesion. PLoS ONE. 2016;11:e0165296.CrossRefGoogle Scholar
  10. 10.
    Yamagami A, Nagaoka N, Yoshihara K, Nakamura M, Shirai H, Matsumoto T, et al. Ultra-structural evaluation of an anodic oxidated titanium dental implant. Dent Mater J. 2014;33:828–34.CrossRefGoogle Scholar
  11. 11.
    Du X, Huang F, Zhang S, Yao Y, Chen Y, Chen Y, et al. Carboxymethylcellulose with phenolic hydroxyl microcapsules enclosinggene-modified BMSCs for controlled BMP-2 release in vitro. Artif Cells Nanomed Biotechnol. 2017;45:1710–20.CrossRefGoogle Scholar
  12. 12.
    Sun P, Wang J, Zheng Y, Fan Y, Gu Z. BMP2/7 heterodimer is a stronger inducer of bone regeneration in peri-implant bone defects model than BMP2 or BMP7 homodimer. Dent Mater J. 2012;31:239–48.CrossRefGoogle Scholar
  13. 13.
    Fernandez-Garcia E, Chen X, Gutierrez-Gonzalez CF, Fernandez A, Lopez-Esteban S, Aparicio C. Peptide-functionalized zirconia and new zirconia/titanium biocermets for dental applications. J Dent. 2015;43:1162–74.CrossRefGoogle Scholar
  14. 14.
    Grober U, Schmidt J, Kisters K. Magnesium in prevention and therapy. Nutrients.2015;7:8199–226.CrossRefGoogle Scholar
  15. 15.
    Fan J, Qiu X, Niu X, Tian Z, Sun W, Liu X, et al. Microstructure, mechanical properties, in vitro degradation and cytotoxicity evaluations of Mg-1.5Y-1.2Zn-0.44Zr alloys for biodegradable metallic implants. Mater Sci Eng C Mater Biol Appl. 2013;33:2345–52.CrossRefGoogle Scholar
  16. 16.
    Huang Z, Wang Z, Li C, Yin K, Hao D, Lan J. Application of plasma sprayed zirconia coating in dental implant: study in implant. J Oral Implantol. 2018. [Epub ahead of print].Google Scholar
  17. 17.
    Guo M, Wang Z, Fan X, Bian Y, Wang T, Zhu L, et al. kgp, rgpA, and rgpB DNA vaccines induce antibody responses in experimental peri-implantitis. J Periodontol. 2014;85:1575–81.CrossRefGoogle Scholar
  18. 18.
    Salvagni E, Berguig G, Engel E, Rodriguez-Cabello JC, Coullerez G, Textor M, et al. A bioactive elastin-like recombinamer reduces unspecific protein adsorption and enhances cell response on titanium surfaces. Colloids Surf B Biointerfaces. 2014;114:225–33.CrossRefGoogle Scholar
  19. 19.
    Fraioli R, Dashnyam K, Kim JH, Perez RA, Kim HW, Gil J, et al. Surface guidance of stem cell behavior: chemically tailored co-presentation of integrin-binding peptides stimulates osteogenic differentiation in vitro and bone formation in vivo. Acta Biomater. 2016;43:269–81.CrossRefGoogle Scholar
  20. 20.
    Olivares-Navarrete R, Hyzy SL, Dunn GR, Almaguer-Flores A, Schwartz Z, Boyan BD, et al. Role of integrin subunits in mesenchymal stem cell differentiation and osteoblast maturation on graphitic carbon-coated microstructured surfaces. Biomaterials. 2015;51:69–79.CrossRefGoogle Scholar
  21. 21.
    Gittens RA, Berner S, Tannenbaum R, Schwartz Z, Sandhage KH, Boyan BD, et al. The effects of combined micron-/submicron-scale surface roughness and nanoscale features on cell proliferation and differentiation. Biomaterials. 2011;32:3395–403.CrossRefGoogle Scholar
  22. 22.
    Wang X, Li S, Yan C, Liu P, Ding J. Fabrication of RGD micro/nanopattern and corresponding study of stem cell differentiation. Nano Lett. 2015;15:1457–67.CrossRefGoogle Scholar
  23. 23.
    Paredes V, Salvagni E, Rodriguez-Castellon E, Gil FJ, Manero JM. Study on the use of 3-aminopropyltriethoxysilane and 3-chloropropyltriethoxysilane to surface biochemical modification of a novel low elastic modulus Ti-Nb-Hf alloy. J Biomed Mater Res B Appl Biomater. 2015;103:495–502.CrossRefGoogle Scholar
  24. 24.
    Felding-Habermann B, Cheresh DA. Vitronectin and its receptors. Curr Opin Cell Biol. 1993;5:864–8.CrossRefGoogle Scholar
  25. 25.
    Mattila PK, Lappalainen P. Filopodia: molecular architecture and cellular functions. Nat Rev Mol Cell Biol. 2008;9:446–54.CrossRefGoogle Scholar
  26. 26.
    Bornschlogl T. How filopodia pull: what we know about the mechanics and dynamics of filopodia. Cytoskelet. 2013;70:590–603.CrossRefGoogle Scholar
  27. 27.
    Brunner M, Nakchbandi IA, Mosher D, Block MR, Albiges-Rizo C, Bouvard D, et al. Osteoblast mineralization requires beta1 integrin/ICAP-1-dependent fibronectin deposition. J Cell Biol. 2011;194:307–22.CrossRefGoogle Scholar
  28. 28.
    Nakayamada S, Okada Y, Saito K, Tamura M, Tanaka Y. Beta1 integrin/focal adhesion kinase-mediated signaling induces intercellular adhesion molecule 1 and receptor activator of nuclear factor kappaB ligand on osteoblasts and osteoclast maturation. J Biol Chem. 2003;278:45368–74.CrossRefGoogle Scholar
  29. 29.
    Huveneers S, Danen EH. Adhesion signaling - crosstalk between integrins, Src and Rho. J Cell Sci. 2009;122:1059–69.CrossRefGoogle Scholar
  30. 30.
    Mavropoulos E, Hausen M, Costa AM, Alves G, Mello A, Ospina CA, et al. The impact of the RGD peptide on osteoblast adhesion and spreading on zinc-substituted hydroxyapatite surface. J Mater Sci Mater Med. 2013;24:1271–83.CrossRefGoogle Scholar
  31. 31.
    Salem AM, Jones SJ, Ellis IR, Chadwick RG. Investigating the addition of collagen and its integrin binding sequence (RGD) to glass polyalkenoate: In terms of material and cellular properties to explore a more biocompatible method of root caries restoration. J Dent. 2016;54:68–76.CrossRefGoogle Scholar
  32. 32.
    Heller M, Kumar VV, Pabst A, Brieger J, Al-Nawas B, Kammerer PW. Osseous response on linear and cyclic RGD-peptides immobilized on titanium surfaces in vitro and in vivo. J Biomed Mater Res A. 2018;106:419–27.CrossRefGoogle Scholar
  33. 33.
    Bilem I, Chevallier P, Plawinski L, Sone ED, Durrieu MC, Laroche G. RGD and BMP-2 mimetic peptide crosstalk enhances osteogenic commitment of human bone marrow stem cells. Acta Biomater. 2016;36:132–42.CrossRefGoogle Scholar
  34. 34.
    Cao FY, Yin WN, Fan JX, Tao L, Qin SY, Zhuo RX, et al. Evaluating the effects of charged oligopeptide motifs coupled with RGD on osteogenic differentiation of mesenchymal stem cells. ACS Appl Mater Interfaces. 2015;7:6698–705.CrossRefGoogle Scholar
  35. 35.
    Pardun K, Streckbein P, Heiss C, Gerlach JW, Maendl S, Rezwan K, et al. Magnesium-containing mixed coatings on zirconia for dental implants: mechanical characterization and in vitro behavior. J Biomater Appl. 2015;30:104–18.CrossRefGoogle Scholar
  36. 36.
    Ghosh-Choudhury N, Abboud SL, Nishimura R, Celeste A, Mahimainathan L, Choudhury GG. Requirement of BMP-2-induced phosphatidylinositol 3-kinase and Akt serine/threonine kinase in osteoblast differentiation and Smad-dependent BMP-2 gene transcription. J Biol Chem. 2002;277:33361–8.CrossRefGoogle Scholar
  37. 37.
    Schmitz C, Perraud AL, Johnson CO, Inabe K, Smith MK, Penner R, et al. Regulation of vertebrate cellular Mg2+ homeostasis by TRPM7. Cell. 2003;114:191–200.CrossRefGoogle Scholar
  38. 38.
    Leem YH, Lee KS, Kim JH, Seok HK, Chang JS, Lee DH. Magnesium ions facilitate integrin alpha 2- and alpha 3-mediated proliferation and enhance alkaline phosphatase expression and activity in hBMSCs. J Tissue Eng Regen Med. 2016;10:E527–36.CrossRefGoogle Scholar
  39. 39.
    You R, Li X, Liu Y, Liu G, Lu S, Li M. Response of filopodia and lamellipodia to surface topography on micropatterned silk fibroin films. J Biomed Mater Res A. 2014;102:4206–12.Google Scholar
  40. 40.
    Zhang W, Li Z, Liu Y, Ye D, Li J, Xu L, et al. Biofunctionalization of a titanium surface with a nano-sawtooth structure regulates the behavior of rat bone marrow mesenchymal stem cells. Int J Nanomed. 2012;7:4459–72.Google Scholar
  41. 41.
    Huang Q, Elkhooly TA, Liu X, Zhang R, Yang X, Shen Z, et al. Effects of hierarchical micro/nano-topographies on the morphology, proliferation and differentiation of osteoblast-like cells. Colloids Surf B Biointerfaces. 2016;145:37–45.CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Zhengfei Huang
    • 1
    • 2
  • Zhifeng Wang
    • 1
    • 3
  • Chuanhua Li
    • 1
    • 2
  • Ning Zhou
    • 1
    • 4
  • Fei Liu
    • 1
    • 2
  • Jing Lan
    • 1
    • 2
    Email author
  1. 1.Shandong Provincial Key Laboratory of Oral Tissue Regeneration, School of StomatologyShandong UniversityJinanChina
  2. 2.Department of Prosthodontics, School of StomatologyShandong UniversityJinanChina
  3. 3.Department of Pediatric Dentistry, School of StomatologyShandong UniversityJinanChina
  4. 4.Department of Orthodontics, School of StomatologyShandong UniversityJinanChina

Personalised recommendations