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FGF adsorbed mesoporous bioactive glass with larger pores in enhancing bone tissue engineering

  • Xiaoyan WangEmail author
  • Qianqian Liu
  • Wei Chen
  • Long Liu
Tissue Engineering Constructs and Cell Substrates Original Research
  • 40 Downloads
Part of the following topical collections:
  1. Tissue Engineering Constructs and Cell Substrates

Abstract

Mesoporous bioactive glass (MBG) is performed as a bone tissue engineering material because of its good bioactivity, biocompatibility and osteoinducion characteristics. Here, we propose MBG with larger pores (MBG-L) adsorbed fibroblast growth factor (FGF) to facilitate osteoblast differentiation and matrix mineralization. Specifically, we observed that MBG-L promotes calcium deposit precipitation in vitro. In addition, adhesion, proliferation, differentiation and matrix mineralization were promoted after osteoblast cultured on MBG-L/FGF. Interestingly, we found that the transcriptional activity of the critical transcription factor Runx2 was increased through MAPK pathway after osteoblast cultured on MBG-L/FGF. Support for this result, we found that the expression of osteoblastic marker genes, Osteocalcin (Ocn), Osteopontin (Opn), and Runx2 were increased. Thus, our findings provided that MBG-L/FGF could be a promising new material in bone tissue engineering.

Notes

Acknowledgements

We thank Dr. RT Franceschi for kindly providing 1.3 kb mOG2-Luc plasmids. This work was supported by the Research Program of National University of Defense Technology (ZK16-03-15) and Nature Science Foundation of Hunan Province (2018JJ3598).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of Biology and Chemistry, College of Liberal Arts and SciencesNational University of Defense TechnologyChangshaP. R. China
  2. 2.Department of Biochemistry, School of Life SciencesCentral South UniversityChangshaP. R. China
  3. 3.Department of Life Sciences, College of Life SciencesHunan Normal UniversityChangshaP. R. China

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