In vitro and in vivo evaluation of chitosan scaffolds combined with simvastatin-loaded nanoparticles for guided bone regeneration
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The objective of this study was to fabricate and characterize chitosan combined with different amounts of simvastatin-loaded nanoparticles and to investigate their potential for guided bone regeneration in vitro and in vivo. Different SIM-CSN formulations were combined into a chitosan scaffold (SIM-CSNs-S), and the morphology, simvastatin release profile, and effect on cell proliferation and differentiation were investigated. For in vivo experiments, ectopic osteogenesis and the critical-size cranial defect model in SD rats were chosen to evaluate bone regeneration potential. All three SIM-CSNs-S formulations had a porous structure and exhibited sustained simvastatin release. CSNs-S showed excellent degradation and biocompatibility characteristics. The 4 mg SIM-CSNs-S formulation stimulated higher BMSC ALP activity levels, demonstrated significantly earlier collagen enhancement, and led to faster bone regeneration than the other formulations. SIM-CSNs-S should have a significant effect on bone regeneration.
Simvastatin-loaded nanoparticles of chitosan.
The preparing process of Chitosan scaffolds combined with simvastatin loaded is simple, low-cost and environmental friendly.
A sustained release of simvastatin.
This work was jointly supported by the National Natural Science Foundation of China (Grant no: 81571016, Grant no: 51272181 and Grant no: 51672030), the science and technology development fund project of Tianjin University (Grant no: 20110410) and the scientific research fund of Stomatology Hospital Affiliated with Tianjin Medical University (Grant no: 2013YKYQ01).
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
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