In vitro and in vivo evaluation of rotary-jet-spun poly(ɛ-caprolactone) with high loading of nano-hydroxyapatite

  • Telmo M. Andrade
  • Daphne C. R. Mello
  • Conceição M. V. Elias
  • Julia M. A. Abdala
  • Edmundo Silva
  • Luana M. R. Vasconcellos
  • Carla R. Tim
  • Fernanda R. Marciano
  • Anderson O. LoboEmail author
Biomaterials Synthesis and Characterization Rapid Communication
Part of the following topical collections:
  1. Biomaterials Synthesis and Characterization


Herein, poly(ɛ-caprolactone) (PCL) mats with different amounts of nanohydroxyapatite (nHAp) were produced using rotary-jet spinning (RJS) and evaluated in vitro and in vivo. The mean fiber diameters of the PCL, PCL/nHAp (3%), PCL/nHAp (5%), and PCL/nHAp (20%) scaffolds were 1847 ± 1039, 1817 ± 1044, 1294 ± 4274, and 845 ± 248 nm, respectively. Initially, all the scaffolds showed superhydrophobic behavior (contact angle around of 140oC), but decreased to 80° after 30 min. All the produced scaffolds were bioactive after soaking in simulated body fluid, especially PCL/nHAp (20%). The crystallinity of the PCL scaffolds decreased progressively from 46 to 21% after incorporation of 20% nHAp. In vitro and in vivo cytotoxicity were investigated, as well as the mats’ ability to reduce bacteria biofilm formation. In vitro cellular differentiation was evaluated by measuring alkaline phosphatase activity and mineralized nodule formation. Overall, we identified the total ideal amount of nHAp to incorporate in PCL mats, which did not show in vitro or in vivo cytotoxicity and promoted lamellar bone formation independently of the amounts of nHAp. The scaffolds with nHAp showed reduced bacterial proliferation. Alizarin red staining was higher in materials associated with nHAp than in those without nHAp. Overall, this study demonstrates that PCL with nHAp prepared by RJS merits further evaluation for orthopedic applications.



AOL and FRM acknowledge the National Council for Scientific and Technological Development (AOL grant: 303752/2017-3 and FRM grant: 304133/2017-5) for support of this research.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Telmo M. Andrade
    • 1
  • Daphne C. R. Mello
    • 2
  • Conceição M. V. Elias
    • 1
  • Julia M. A. Abdala
    • 3
  • Edmundo Silva
    • 2
  • Luana M. R. Vasconcellos
    • 2
  • Carla R. Tim
    • 1
  • Fernanda R. Marciano
    • 1
  • Anderson O. Lobo
    • 1
    • 4
    Email author
  1. 1.Instituto Científico e TecnológicoUniversidade Brasil, ItaqueraSão PauloBrazil
  2. 2.Departamento de Biociência e Diagnóstico Oral, Instituto de Ciência e TecnologiaUniversidade Estadual de São PauloSão PauloBrazil
  3. 3.Instituto de Pesquisa e DesenvolvimentoUniversidade do Vale do ParaibaSão PauloBrazil
  4. 4.LIMAV-Laboratório Interdisciplinar de Materiais Avançados, PPGCM-Programa de Pós-graduação em Ciência e Engenharia de MateriaisUFPI-Universidade Federal do PiauíTeresinaBrazil

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