Preparation of a tumor-targeted drug-loading material, amphiphilic peptide P10, and analysis of its anti-tumor activity

  • Fei Ge
  • Qianqian Qiao
  • Longbao Zhu
  • Wanzhen Li
  • Ping Song
  • Longlong Zhu
  • Yugui TaoEmail author
  • Lin GuiEmail author
Delivery Systems Original Research
Part of the following topical collections:
  1. Delivery Systems


A new tumor-targeted drug-loading material, the amphiphilic peptide DGRGGGAAAA (P10) was designed and synthesized, and its self-assembly behavior, drug-loading effects and in vitro characteristics were studied. P10 was synthesized by solid-state synthesis and doxorubicin (DOX) was loaded via dialysis. P10 and DOX were mixed with a mass ratio of 6:1 to form regular round spheres. The interconnection between groups was analyzed spectroscopically and the sphere morphology was studied with SEM and a zeta particle size analyzer. Fluorescence spectroscopy was used to analyze the ability of P10 to form micelles and the efficiency of micelle entrapment, and the drug-loading ratio and drug release characteristics were detected. Finally, the in vitro antitumor activity of P10 was studied with HeLa cells as a model. The results showed that P10’s critical micelle concentration (CMC) value and its average grain diameter were approximately 0.045 mg/L and 500 nm. The micelle entrapment ratio and drug-loading ratio were 23.011 ± 2.88 and 10.125 ± 2.62%, respectively, and the in vitro drug-releasing properties of P10 were described by the Zero-order model and the Ritger-Peppas model. Compared with DOX, P10-DOX had a higher tumor cell inhibition ratio and a dose-effect relationship with concentration. When P10-DOX’s concentration was 20 μg/mL, the inhibition ratio was 44.17%. The new amphiphilic peptide designed and prepared in this study could be a tumor-targeted drug-loading material with better prospects for application.

In this paper, a new tumor-targeted drug-loading material, the amphiphilic peptide DGRGGGAAAA (P10) is designed and synthesized, and its self-assembly behavior, drug-loading effects and in vitro characteristics are studied, providing a theoretical basis and design ideas for further studies and the development of targeted drug-loading materials on tumor cells.



This work was supported by the National Natural Science Foundation of China (NSFC) [grant number 31671797], Natural Science Foundation of Anhui University [grant number 2017KJA123, KJ2017A251 and KJ2018A0117], Anhui Natural Science Foundation [grant number 1808085QC85] and the Youth talent support program of Anhui Polytechnic University [grant number 2016BJRRC006].

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

10856_2018_6204_MOESM1_ESM.docx (29 kb)
Supplementary Information


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© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Biological and Chemical Engineering CollegeAnhui Polytechnic UniversityWuhuChina
  2. 2.Department of Microbiology and immunologyWannan Medical CollegeWuhuChina

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