Cell laden alginate-keratin based composite microcapsules containing bioactive glass for tissue engineering applications
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Microcapsules based on alginate-keratin, alginate dialdehyde (ADA)-keratin and ADA-keratin-45S5 bioactive glass (BG) were successfully prepared. The samples were characterized by light microscopy, scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy (FTIR). The results showed that ADA-based materials possess higher degradation rate compared to alginate–based materials. The incorporation of BG particles (mean particle size: 2.0 µm) improved the bioactivity of the materials. Moreover, the biological properties of the samples were evaluated by encapsulating MG-63 osteosarcoma cells into the microcapsules. The cell viability in all samples increased during 21 days of cultivation. However, the presence of 0.5% BG particle seemed to have initial negative effect on cell growth compared to other samples without BG. On the other hand, the positive effect of CaP formation was visible after 3 weeks in the BG containing samples. The results are relevant to consider the development of cell laden bioinks incorporating inorganic bioactive particles for biofabrication approaches.
Supachai Reakasame acknowledges the German Academic Exchange Service (DAAD) for financial support. We thank Dr.-Ing. Kai Zheng and Dr. Aldo Leal-Egaña (Institute of Biomaterials, University of Erlangen-Nuremberg) for helpful discussions.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
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