Defragmentation of lysozyme derived Amyloid β fibril using Biocompatible Magnetic fluid
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We present here a modulating effect on lysozyme derived Amyloid β fibrils by aqueous magnetic fluid. This non-conventional approach of treatment of lysozyme derived Amyloid β fibrils showed lysing of Amyloid fibrils to its secondary structures which can be seen using optical microscope and scanning electron microscopic image. The size of lysozyme derived amyloid fibrils before and after treatment was measured using dynamic light scattering technique. The mechanism of defragmentation of lysozyme derived Amyloid β fibrils by magnetic fluid is explained. This is a first report to identify the secondary structure of protein using Fourier Transform Infrared (FTIR) and Circular Dichroism (CD) spectra after lysing. The cyto-toxicity study of this magnetic fluid on neuronal (SH-SY5Y) and non-neuronal (NRK) cell lines shows non-toxicity up to a concentration of 250 μg/mL. The study indicates a novel and unique complementary approach to treat the amyloidogenic brain diseases.
Authors would like to thank DST, Govt. of India for providing financial support to NP under technology development project no. 161-G and CHARUSAT for providing experimental facilities.
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Conflict of interest
The authors declare that they have no conflict of interest.
- 22.Martin SR, Bayley PM.Calcium-Bind Protein Protoc: Methods Tech. Springer Publications, 2002;2:43Google Scholar
- 30.Joachim E, Kim ID, Jin Y, Kim KK, Lee JK, Choi H. Gelatin nanoparticles enhance the neuroprotective effects of intranasally administered osteopontin in rat ischemic stroke model. Drug Deliv Transl Res. 2014;395:4.5–6Google Scholar