Fabrication of enzyme-responsive composite coating for the design of antibacterial surface

  • Peng LiuEmail author
  • Yansha Hao
  • Yao Ding
  • Zhang Yuan
  • Yisi Liu
  • Kaiyong CaiEmail author
Biomaterials Synthesis and Characterization Original Research
Part of the following topical collections:
  1. Biomaterials Synthesis and Characterization


In this study, a type of bacteria enzyme-triggered antibacterial surface with a controlled release of Ag ions was developed. Firstly, chitosan-silver nanocomposites (Chi@Ag NPs) were in situ synthesized via using ascorbic acid as reducing agent. Chi@Ag NPs were characterized by transmission electron microscopy, ultraviolet–visible spectroscopy, X-ray diffraction and X-ray photoelectron spectroscopy. Subsequently, Chi@Ag NPs and hyaluronic acid (HA) were used to fabricate antibacterial composite coating via Layer-by-Layer (LBL) self-assembly method. The successful construction of Chi@Ag NPs/HA composite coating was confirmed by scanning electron microscopy, energy dispersive spectroscopy and contact angle measurements, respectively. Then, the amount of released Ag ion was analyzed by inductively coupled plasma atomic emission spectrometry, which demonstrated that the release of Ag ions from the surface could be triggered by enzyme (e.g. hyaluronidase). A series of antibacterial tests in vitro, including zone of inhibition test, bacterial viability assay, antibacterial rate measurement and bacteria adhesion observation, demonstrated that the enzyme-responsive surface could inhibit the growth of bacteria. On the whole, this study provides an alternative approach for the fabrication of antibacterial surfaces on synthetic materials in various fields with the minimal side effects on surrounding environment and human body.



This work was financially supported by State Key Project of Research and Development (Project No. 2016YFC1100300), Natural Science Foundation of China (Project Nos. 51303218, 51673032 and 21734002), Fundamental Research Funds for the Central Universities (Project No. 2018CDXYSW0023), Chongqing Research Program of Technological Innovation and Application Demonstration (Project No. cstc2018jscx-msybX0299) and Innovation Team in University of Chongqing Municipal Government (Project No. CXTDX201601002).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.


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© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Key Laboratory of Biorheological Science and Technology of Ministry of Education, College of BioengineeringChongqing UniversityChongqingChina

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