In vitro evaluation of a multispecies oral biofilm over antibacterial coated titanium surfaces
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Peri-implantitis is an infectious disease that affects the supporting soft and hard tissues around dental implants and its prevalence is increasing considerably. The development of antibacterial strategies, such as titanium antibacterial-coated surfaces, may be a promising strategy to prevent the onset and progression of peri-implantitis. The aim of this study was to quantify the biofilm adhesion and bacterial cell viability over titanium disc with or without antibacterial surface treatment. Five bacterial strains were used to develop a multispecies oral biofilm. The selected species represent initial (Streptococcus oralis and Actinomyces viscosus), early (Veillonella parvula), secondary (Fusobacterium nucleatum) and late (Porphyromonas gingivalis) colonizers. Bacteria were sequentially inoculated over seven different types of titanium surfaces, combining different roughness level and antibacterial coatings: silver nanoparticles and TESPSA silanization. Biofilm formation, cellular viability and bacterial quantification over each surface were analyzed using scanning electron microscopy, confocal microscopy and real time PCR. Biofilm formation over titanium surfaces with different bacterial morphologies could be observed. TESPSA was able to significantly reduce the cellular viability when compared to all the surfaces (p < 0.05). Silver deposition on titanium surface did not show improved results in terms of biofilm adhesion and cellular viability when compared to its corresponding non-coated surface. The total amount of bacterial biofilm did not significantly differ between groups (p > 0.05). TESPSA was able to reduce biofilm adhesion and cellular viability. However, silver deposition on titanium surface seemed not to confer these antibacterial properties.
Dr Nart, Dr Delgado and Mr Vilarrasa from UIC Barcelona would also like to acknowledge the Dentaid Research Centre (Dentaid S.L.) for the technical support and the access to their facilities. The work was supported by Klockner Implant System.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
- 2.Albrektsson T, Zarb G, Worthington P, Eriksson AR. The long-term efficacy of currently used dental implants: a review and proposed criteria of success. Int J Oral Maxillofac Implants. 1986;1:11–25.Google Scholar
- 4.Buser D, Chappuis V, Bornstein MM, Wittneben JG, Frei M, Belser UC. Long-term stability of contour augmentation with early implant placement following single tooth extraction in the esthetic zone: a prospective, cross-sectional study in 41 patients with a 5- to 9-year follow-up. J Periodontol. 2013;84:1517–27.Google Scholar
- 12.Godoy-Gallardo M, Guillem-Marti J, Sevilla P, Manero JM, Gil FJ, Rodriguez D. Anhydride-functional silane immobilized onto titanium surfaces induces osteoblast cell differentiation and reduces bacterial adhesion and biofilm formation. Mater Sci Eng C Mater Biol Appl. 2016;59:524–32.CrossRefGoogle Scholar
- 18.Wu-Yuan CD, Eganhouse KJ, Keller JC, Walters KS. Oral bacterial attachment to titanium surfaces: a scanning electron microscopy study. J Oral Implantol. 1995;21:207–13.Google Scholar
- 19.Drake DR, Paul J, Keller JC. Primary bacterial colonization of implant surfaces. Int J Oral Maxillofac Implants. 1999;14:226–32.Google Scholar
- 26.Godoy-Gallardo M, Rodríguez-Hernández AG, Delgado LM, Manero JM, Javier Gil F, Rodríguez D. Silver deposition on titanium surface by electrochemical anodizing process reduces bacterial adhesion of Streptococcus sanguinis and Lactobacillus salivarius. Clin Oral Implants Res. 2015;26:1170–9.CrossRefGoogle Scholar