In vitro evaluation of a multispecies oral biofilm over antibacterial coated titanium surfaces

  • Javi Vilarrasa
  • Luis M Delgado
  • Marta Galofré
  • Gerard Àlvarez
  • Deborah Violant
  • José María Manero
  • Vanessa Blanc
  • F. Javier Gil
  • José NartEmail author
Biocompatibility Studies Original Research
Part of the following topical collections:
  1. Biocompatibility Studies


Peri-implantitis is an infectious disease that affects the supporting soft and hard tissues around dental implants and its prevalence is increasing considerably. The development of antibacterial strategies, such as titanium antibacterial-coated surfaces, may be a promising strategy to prevent the onset and progression of peri-implantitis. The aim of this study was to quantify the biofilm adhesion and bacterial cell viability over titanium disc with or without antibacterial surface treatment. Five bacterial strains were used to develop a multispecies oral biofilm. The selected species represent initial (Streptococcus oralis and Actinomyces viscosus), early (Veillonella parvula), secondary (Fusobacterium nucleatum) and late (Porphyromonas gingivalis) colonizers. Bacteria were sequentially inoculated over seven different types of titanium surfaces, combining different roughness level and antibacterial coatings: silver nanoparticles and TESPSA silanization. Biofilm formation, cellular viability and bacterial quantification over each surface were analyzed using scanning electron microscopy, confocal microscopy and real time PCR. Biofilm formation over titanium surfaces with different bacterial morphologies could be observed. TESPSA was able to significantly reduce the cellular viability when compared to all the surfaces (p < 0.05). Silver deposition on titanium surface did not show improved results in terms of biofilm adhesion and cellular viability when compared to its corresponding non-coated surface. The total amount of bacterial biofilm did not significantly differ between groups (p > 0.05). TESPSA was able to reduce biofilm adhesion and cellular viability. However, silver deposition on titanium surface seemed not to confer these antibacterial properties.



Dr Nart, Dr Delgado and Mr Vilarrasa from UIC Barcelona would also like to acknowledge the Dentaid Research Centre (Dentaid S.L.) for the technical support and the access to their facilities. The work was supported by Klockner Implant System.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of Periodontology Faculty of DentistryUniversitat Internacional de CatalunyaBarcelonaSpain
  2. 2.Bioengineering Institute of TechnologyUniversitat Internacional de CatalunyaBarcelonaSpain
  3. 3.Department of MicrobiologyDentaid Research CenterBarcelonaSpain
  4. 4.Faculty of DentistryUniversitat Internacional de CatalunyBarcelonaSpain
  5. 5.Biomaterials, Biomechanics and Tissue Engineering Group (BBT)Department of Materials Science and Metallurgical Engineering Technical University of Catalonia (UPC)BarcelonaSpain

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