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Restoration of mandibular bone defects with demineralized bone matrix combined with three-dimensional cultured bone marrow-derived mesenchymal stem cells in minipig models

  • Clinical Applications of Biomaterials
  • Original Research
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Abstract

Mandibular defects, caused by congenital, pathological or iatrogenic insults, can significantly affect patient quality of life. The reconstruction of mandible has recently gained the interest of clinical and tissue engineering researchers. The purpose of this study was to evaluate the effectiveness of three-dimensional (3-D) cultured autologous grafts prepared using bone marrow-derived mesenchymal stem cells (BMSCs) combined with demineralized bone matrix (DBM) scaffolds for the restoration of mandibular defects. Cylindrical defects were created in the mandibular body of minipigs and filled with 3D-cultured BMSCs/DBM autografts, 2D-cultured BMSCs/DBM autografts, DBM material (without cells), or were left unfilled (blank). Using computed tomographic (CT) imaging and histological staining, we found that treatment of mandibular defects using 3-D cultured BMSCs/DBM autografts offered improvements in bone formation over both 2-D cultured autografts and cell-free DBM scaffolds. We found increased osteoid formation in 3D and 2D cultures, with more osteogenic cells present in the 3D constructs. We suggest that 3-D cultured homograft BMSCs combined with DBM scaffolds represents a new strategy for bone reconstruction, with potential future clinical applicability.

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Acknowledgements

This work was supported by National Key R&D Program of China (2016YFC1000800, 2016YFC1000806, 2016YFC1101500, 2017YFA0104700), National Research Institute for Family Planning (2016GJZ06), the National Science Foundation of China (81601084), Strategic Priority Research Program of the Chinese Academy of Sciences (XDA04020000). We thank Rebecca Jackson, PhD, from Liwen Bianji, Edanz Group China (www.liwenbianji.cn/ac), for editing the English text of a draft of this manuscript.

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Author notes

  1. These authors contributed equally: Jianwu Dai, Chao Lu and Yi Cui.

Authors and Affiliations

  1. Reproductive and Genetic Center of National Research Institute for Family Planning, Beijing, 100081, P.R. China

    Yi Cui

  2. Department of Oral & Maxillofacial Surgery, Beijing Chongwen Hospital of Stomatology, Beijing, 100062, China

    Chao Lu

  3. Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, 100080, China

    Bing Chen, Jin Han, Yannan Zhao, Zhifeng Xiao, Sufang Han & Jianwu Dai

  4. Department of VIP Service, School of Stomatology, Capital Medical University, Beijing, China

    Juli Pan

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  1. Yi Cui
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Correspondence to Juli Pan or Jianwu Dai.

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Cui, Y., Lu, C., Chen, B. et al. Restoration of mandibular bone defects with demineralized bone matrix combined with three-dimensional cultured bone marrow-derived mesenchymal stem cells in minipig models. J Mater Sci: Mater Med 29, 147 (2018). https://doi.org/10.1007/s10856-018-6152-3

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  • DOI: https://doi.org/10.1007/s10856-018-6152-3

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