Effect of strontium substitution on the cytocompatibility and 3-D scaffold structure for the xSrO–(10−x) MgO–60SiO2–20CaO–10 P2O5 (2 ≤ x ≤ 8) sol–gel glasses
- 120 Downloads
In the present study, novel glasses xSrO–(10−x) MgO–60SiO2–20CaO–10 P2O5 (2 ≤ x ≤ 8, in steps of 2) are synthesized via sol–gel method. The current work focusses on the evaluation of mechanical, physical and biocompatible properties for sol–gel glasses. The pore size and surface area of these glasses were studied using BET analysis. The structural aspect of the glasses/glass ceramics was studied by XRD and Raman spectroscopy. The cytotoxicity assays were conducted for MG63 human osteosarcoma cell line. Furthermore, the as prepared glasses were used for the fabrication of 3-D porous scaffolds via polymer replication method. The loaded green bodies have been sintered at 700, 800 and 900 °C and were kept for 6 h to densify the glass network. The effect of sintering temperature on the structure and properties of as prepared scaffolds were analyzed via scanning electron microscopy (SEM) and porosity calculations.
KeywordsFracture Toughness Strontium Sinter Temperature Glass Sample Bioactive Glass
The authors are thankful to Mr. Aayush Gupta for his valuable suggestions. One of the author GK is thankful to University Grant Commission (UGC) under the letter no. F 15/2013-2014/PDFWM – 2013-2014-GE – PUN – 14803 (SA-II) for providing financial assistance.
Conflict of interest
The authors declare that they have no competing interests.
- 11.Kaur G, Pandey OP, Singh K, Homa D, Scott B, Pickrell G. A review of bioactive glasses: their structure, properties, fabrication and apatite formation. J Biomed Mater Res A 2013; 254-74.Google Scholar
- 12.Hench LL, Splinter RJ, Allen WC, Greenlee TK. Bonding mechanisms at the interface of ceramic prosthetic materials. J Biomed Mater Res. 1972;2:117–41.Google Scholar
- 18.Brovarone CV, Verné E, Appendino P. Macroporous bioactive glass-ceramic scaffolds for tissue engineering. J Mater Sci: Mater Med. 2006;17:1069–78.Google Scholar
- 24.Kaur G, Pickrell G, Kimsawatde G, Homa D, Allbee HA, Sriranganathan N. Synthesis, cytotoxicity, and hydroxyapatite formation in 27-Tris-SBF for sol gel based CaO-P2O5-SiO2-B2O3-ZnO bioactive glasses. Sci Rep. 2014;4:1–14.Google Scholar
- 25.Kaur G, Pandey OP, Chudasama BN, Kumar V. Combined and individual doxorubicin/vancomycin drug loading, release kinetics and apatite formation for the CaO – CuO – P2O5 – SiO2 – B2O5 mesoporous glasses. RSC Adv 2016; 51046–56.Google Scholar
- 29.Sriranganathan D, Kanwal N, Hing KA, Hill RG. Strontium substituted bioactive glasses for tissue engineered scaffolds: the importance of octacalcium phosphate. J Mater Sci: Mater Med. 2016;27:1–10.Google Scholar
- 32.Gorustovich AA, Steimetz T, Cabrini RL, Porto Lopez JM. Osteoconductivity of strontium-doped bioactive glass particles: a histomorphometric study in rats. Soc Biomater. 2010;92A:232–7.Google Scholar
- 41.O’ Donnell MD, Watts SJ, Hill RG, Law RV. The effect of phosphate content on bioactivity of soda lime phosphosilicate glasses. J Mater Sci: Mater Med. 2009;20:1611–8.Google Scholar
- 49.Bretcanu O, Chen Q, Misra SK, Boccaccini AR, Verne E, Vitale Brovarone C. Biodegradable polymer coated 45S5 bioglass derived glass-ceramic scaffolds for bone tissue engineering. Glass Tech: Eur J Glass Sci Tech A. 2007;48:227–34.Google Scholar
- 50.Mencik J. Strength and fracture of glass ceramics. Glass Sci and Tech 12 (1992). Amsterdam:Elsevier.Google Scholar
- 55.Ranjan A, Pothayee N, Seleem MN, Tyler RD, Brenseke B, Sriranganathan N, Riffle JS, Kasimanickam R. Antibacterial efficacy of core-shell nanostructures encapsulating gentamicin against an in, vivo intracellular Salmonella model. Int J Nanomed 2009; 4:289–97.Google Scholar
- 58.Kuboki Y, Jin Q, Takita H. Geometry of carriers controlling phenotypic expression in BMP-induced osteogenesis and chondrogenesis. J Bone Joint Surg Am. 2001;83-A:105–15.Google Scholar
- 59.Garg S, Thakur S, Gupta A, Kaur G, Pandey OP. Antibacterial and anticancerous drug loading kinetics for (10-x) CuO-xZnO-20CaO-60SiO2-10P2O5 (2 ≤ x ≤ 8) mesoporous bioactive glasses. J Mater Sci: Mater Med. 2017;28:1–14.Google Scholar