Green synthesis of rifampicin-loaded copper nanoparticles with enhanced antimicrobial activity
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The antimicrobial properties of copper and rifampicin-loaded copper nanoparticles were investigated using four strains: Staphylococcus aureus, Escherichia coli, Bacillus pumilis and Pseudomonas fluorescens. Spherical-shaped copper nanoparticles were synthesized via green reduction method from the peppermint extract. It was found that adsorption of rifampicin on the copper nanosurface enhances its biological activity and prevents the development of resistance. The interactions between rifampicin-copper nanoparticles and bacteria cells were monitored using atomic force microscopy (AFM) and confocal laser scanning microscopy (CLSM). It was proven that loaded with rifampicin copper nanoparticles were able to damage the S. aureus cell membrane and facilitate the bacteria biofilm matrix disintegration. Moreover, the DNA decomposition of S. aureus treated with copper and rifampicin-copper nanoparticles was confirmed by agarose gel electrophoresis. The results obtained indicate that adsorption of rifampicin on the copper nanoparticles surface might provide the reduction of antibiotic dosage and prevent its adverse side effects.
- 3.Landers TF, Cohen B, Wittum TE, Larson EL. A review of antibiotic use in food animals: perspective, policy, and potential. Public Health Rep. 2012;127:4–22.Google Scholar
- 5.Usman MS, Zowalaty MEE, Shameli K, Zainuddin N, Salama M, Ibrahim NA. Synthesis, characterization, and antimicrobial properties of copper nanoparticles. Int J Nanomedicine. 2013;8:4467–79.Google Scholar
- 25.Kaczmarska L, Jakoniuk P. Therapeutic effect of some antibiotics on experimental staphylococcal infection and its correlation with in vitro activity of antibiotics in sub-inhibitory concentration against Staphylococcus aureus strains. Med Dosw Mikrobiol. 2003;55:1–10.Google Scholar
- 29.Parsons J, Peralta-Videa J, Gardea-Torresdey J. Use of plants in biotechnology: synthesis of metal nanoparticles by inactivated plant tissues, plant extracts, and living plants. Rev Environ Sci. 2007;5:463–85.Google Scholar
- 31.Hughes D, Andersson DI. Antibiotic development and resistance. NewYork, NY: Taylor and Francis Inc; 2001.Google Scholar
- 38.Lyubchik S, Lyubchik A, Lygina O, Lyubchik S, Fonseca I. (editors) Comparison of the thermodynamic parameters estimation for the adsorption process of the metals from liquid phase on activated carbons. Thermodynamics—interaction studies—solids, liquids and gases. InTech. 2011. Available from: http://www.intechopen.com/books/thermodynamics-interaction-studies-solids-liquids-and-gases/comparison-of-the-thermodynamic-parameters-estimation-for-the-adsorption-process-of-the-metals-from-.
- 42.Subhankari I, Nayak PL. Antimicrobial activity of copper nanoparticles synthesised by ginger (Zingiber officinale) extract. World J Nucl Sci Technol. 2013;2:10–3.Google Scholar
- 43.Jorgensen JH, Ferraro MJ. Antimicrobial susceptibility testing: a review of general principles and contemporary practices. Med Microbiol. 2009;49:1749–55.Google Scholar
- 44.Suleiman M, Al-Masri M, Al-Ali A, Aref D, Hussein A, Saadeddin I, Warad I. Synthesis of nano-sized sulfur nanoparticles and their antibacterial activities. J Mater Environ Sci. 2015;6:513–8.Google Scholar
- 46.Singhal SK, Lal M, Kabi SR, Mathur RB. Synthesis of Cu/CNTs nanocomposites for antimicrobial activity. Adv Nat Sci: Nanosci Nanotechnol. 2012;3:045011Google Scholar
- 48.Buss WC, Reyes E, Barela TD. Metal ion catalyzed oxidation of the antibiotic rifampicin. Res Commun Chem Pathol Pharmacol. 1977;17:547–609.Google Scholar