Plasma sprayed cerium oxide coating inhibits H2O2-induced oxidative stress and supports cell viability

  • Kai Li
  • Youtao Xie
  • Mingyu You
  • Liping Huang
  • Xuebin Zheng
Biocompatibility Studies Original Research
First Online:
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Part of the following topical collections:
  1. Biocompatibility Studies

Abstract

Oxidative stress is a risk factor in the pathogenesis of osteoporosis, and plays a major role in bone regeneration of osteoporotic patients. Cerium oxide (CeO2) ceramics have the unique ability to protect various types of cells from oxidative damage, making them attractive for biomedical applications. In this study, we developed a plasma sprayed CeO2 coating with a hierarchical topography where ceria nanoparticles were superimposed in the micro-rough coating surface. The protective effects of the CeO2 coating on the response of osteoblasts to H2O2-induced oxidative stress have been demonstrated in terms of cell viability, apoptosis and differentiation. The CeO2 coating reversed the reduced superoxide dismutase activity, decreased reactive oxygen species production and suppressed malondialdehyde formation in H2O2-treated osteoblasts. It indicated that the CeO2 coating can preserve the intracellular antioxidant defense system. The cytocompatibility of the CeO2 coating was further assessed in vitro by cell viability assay and scanning electron microscopy analysis. Taken together, the CeO2 coating could provide an opportunity to be utilized as a potential candidate for bone regeneration under oxidative stress.

Keywords

CeO2 H2O2 Group Intracellular Reactive Oxygen Species Generation Mixed Valence State Predetermined Time Point 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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Notes

Acknowledgments

This work was supported by the National Natural Science Foundation of China (Grant Nos. 51502328, 81301537, 81300917).

Supplementary material

10856_2016_5710_MOESM1_ESM.tif (2.3 mb)
Supplementary material 1 (TIFF 2347 kb)

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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  • Kai Li
    • 1
  • Youtao Xie
    • 1
  • Mingyu You
    • 1
  • Liping Huang
    • 1
  • Xuebin Zheng
    • 1
  1. 1.Key Laboratory of Inorganic Coating Materials, Shanghai Institute of CeramicsChinese Academy of SciencesShanghaiPeople’s Republic of China

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