Multiwall carbon nanotubes/polycaprolactone scaffolds seeded with human dental pulp stem cells for bone tissue regeneration

  • M. L. Flores-Cedillo
  • K. N. Alvarado-Estrada
  • A. J. Pozos-Guillén
  • J. S. Murguía-Ibarra
  • M. A. Vidal
  • J. M. Cervantes-Uc
  • R. Rosales-Ibáñez
  • J. V. Cauich-Rodríguez
Engineering and Nano-engineering Approaches for Medical Devices Original Research
Part of the following topical collections:
  1. Engineering and Nano-engineering Approaches for Medical Devices

Abstract

Conventional approaches to bone regeneration rarely use multiwall carbon nanotubes (MWCNTs) but instead use polymeric matrices filled with hydroxyapatite, calcium phosphates and bioactive glasses. In this study, we prepared composites of MWCNTs/polycaprolactone (PCL) for bone regeneration as follows: (a) MWCNTs randomly dispersed on PCL, (b) MWCNTs aligned with an electrical field to determine if the orientation favors the growing of human dental pulp stem cells (HDPSCs), and (c) MWCNTs modified with β-glycerol phosphate (BGP) to analyze its osteogenic potential. Raman spectroscopy confirmed the presence of MWCNTs and BGP on PCL, whereas the increase in crystallinity by the addition of MWCNTs to PCL was confirmed by X-ray diffraction and differential scanning calorimetry. A higher elastic modulus (608 ± 4.3 MPa), maximum stress (42 ± 6.1 MPa) and electrical conductivity (1.67 × 10−7 S/m) were observed in non-aligned MWCNTs compared with the pristine PCL. Cell viability at 14 days was similar in all samples according to the live/dead assay, but the 21 day cell proliferation, measured by MTT was higher in MWCNTs aligned with BGP. Von Kossa and Alizarin red showed larger amounts of mineral deposits on MWCNTs aligned with BGP, indicating that at 21 days, this scaffold promotes osteogenic differentiation of HDPSCs.

Supplementary material

Supplementary material 1 (AVI 1848 kb)

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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  • M. L. Flores-Cedillo
    • 1
  • K. N. Alvarado-Estrada
    • 1
  • A. J. Pozos-Guillén
    • 1
  • J. S. Murguía-Ibarra
    • 2
  • M. A. Vidal
    • 3
  • J. M. Cervantes-Uc
    • 4
  • R. Rosales-Ibáñez
    • 1
  • J. V. Cauich-Rodríguez
    • 4
  1. 1.Facultad de Estomatología, Laboratorio de Ciencias BásicasUniversidad Autónoma de San Luis PotosíSan Luis PotosíMexico
  2. 2.Facultad de Ciencias, Departamento de ElectrónicaUniversidad Autónoma de San Luis PotosíSan Luis PotosíMexico
  3. 3.Centro de Aplicación de Radiación Infrarroja, Energías Alternativas y MaterialesCIACYTSan Luis PotosíMexico
  4. 4.Centro de Investigación Científica de YucatánUnidad de MaterialesMéridaMexico

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