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Degradation and silicon excretion of the calcium silicate bioactive ceramics during bone regeneration using rabbit femur defect model

  • Kaili Lin
  • Yong Liu
  • Hai Huang
  • Lei Chen
  • Zhen WangEmail author
  • Jiang ChangEmail author
Biocompatibility Studies
Part of the following topical collections:
  1. Biocompatibility Studies

Abstract

The investigation of the bone regeneration ability, degradation and excretion of the grafts is critical for development and application of the newly developed biomaterials. Herein, the in vivo bone-regeneration, biodegradation and silicon (Si) excretion of the new type calcium silicate (CaSiO3, CS) bioactive ceramics were investigated using rabbit femur defect model, and the results were compared with the traditional β-tricalcium phosphate [β-Ca3(PO4)2, β-TCP] bioceramics. After implantation of the scaffolds in rabbit femur defects for 4, 8 and 12 weeks, the bone regenerative capacity and degradation were evaluated by histomorphometric analysis. While urine and some organs such as kidney, liver, lung and spleen were resected for chemical analysis to determine the excretion of the ionic products from CS implants. The histomorphometric analysis showed that the bioresorption rate of CS was similar to that of β-TCP in femur defect model, while the CS grafts could significantly stimulate bone formation capacity as compared with β-TCP bioceramics (P < 0.05). The chemical analysis results showed that Si concentration in urinary of the CS group was apparently higher than that in control group of β-TCP. However, no significant increase of the Si excretion was found in the organs including kidney, which suggests that the resorbed Si element is harmlessly excreted in soluble form via the urine. The present studies show that the CS ceramics can be used as safe, bioactive and biodegradable materials for hard tissue repair and tissue engineering applications.

Keywords

Calcium Silicate Silicon Concentration Calcium Silicate Calvarial Defect Implantation Period 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

This work was supported by grants from the Natural Science Foundation of China (Grant No.: 30900299, 81190132 and 51061160499).

References

  1. 1.
    Lin K, Xia L, Li H, Jiang X, Pan H, Xu Y, et al. Enhanced osteoporotic bone regeneration by strontium-substituted calcium silicate bioactive ceramics. Biomaterials. 2013;34:10028–42.CrossRefGoogle Scholar
  2. 2.
    Xu S, Lin K, Wang Z, Chang J, Wang L, Lu J, et al. Reconstruction of calvarial defect of rabbits using porous calcium silicate bioactive ceramics. Biomaterials. 2008;29:2588–96.CrossRefGoogle Scholar
  3. 3.
    Wang C, Lin K, Chang J, Sun J. Osteogenesis and angiogenesis induced by porous beta-CaSiO(3)/PDLGA composite scaffold via activation of AMPK/ERK1/2 and PI3K/Akt pathways. Biomaterials. 2013;34:64–77.CrossRefGoogle Scholar
  4. 4.
    Liu S, Jin F, Lin K, Lu J, Sun J, Chang J, et al. The effect of calcium silicate on in vitro physiochemical properties and in vivo osteogenesis, degradability and bioactivity of porous beta-tricalcium phosphate bioceramics. Biomed Mater. 2013;8:025008.CrossRefGoogle Scholar
  5. 5.
    Lin K, Zhang M, Zhai W, Qu H, Chang J. Fabrication and characterization of hydroxyapatite/wollastonite composite bioceramics with controllable properties for hard tissue repair. J Am Ceram Soc. 2011;94:99–105.CrossRefGoogle Scholar
  6. 6.
    Wang C, Chiang T, Chang H, Ding S. Physicochemical properties and osteogenic activity of radiopaque calcium silicate–gelatin cements. J Mater Sci Mater Med. 2014;25:2193–203.CrossRefGoogle Scholar
  7. 7.
    Motisuke M, Santos VR, Bazanini NC, Bertran CA. Apatite bone cement reinforced with calcium silicate fibers. J Mater Sci Mater Med. 2014;25:2357–63.CrossRefGoogle Scholar
  8. 8.
    Fei L, Wang C, Xue Y, Lin K, Chang J, Sun J. Osteogenic differentiation of osteoblasts induced by calcium silicate and calcium silicate/beta-tricalcium phosphate composite bioceramics. J Biomed Mater Res. 2012;100B:1237–44. CrossRefGoogle Scholar
  9. 9.
    Wang C, Xue Y, Lin K, Lu J, Chang J, Sun J. The enhancement of bone regeneration by a combination of osteoconductivity and osteostimulation using beta-CaSiO3/beta-Ca3(PO4)2 composite bioceramics. Acta Biomater. 2012;8:350–60.CrossRefGoogle Scholar
  10. 10.
    Wang C, Lin K, Chang J, Sun J. The stimulation of osteogenic differentiation of mesenchymal stem cells and vascular endothelial growth factor secretion of endothelial cells by beta-CaSiO3/beta-Ca3(PO4)2 scaffolds. J Biomed Mater Res. 2014;102A:2096–104.CrossRefGoogle Scholar
  11. 11.
    Lin K, Chang J, Shen R. The effect of powder properties on sintering, microstructure, mechanical strength and degradability of beta-tricalcium phosphate/calcium silicate composite bioceramics. Biomed Mater. 2009;4:065009.CrossRefGoogle Scholar
  12. 12.
    Lin K, Zhai W, Ni S, Chang J, Zeng Y, Qian W. Study of the mechanical property and in vitro biocompatibility of CaSiO3 ceramics. Ceram Int. 2005;31:323–6.CrossRefGoogle Scholar
  13. 13.
    Lin K, Chang J, Wang Z. Fabrication and the characterisation of the bioactivity and degradability of macroporous calcium silicate bioceramics in vitro. J Inorg Mater. 2005;20:692–8.Google Scholar
  14. 14.
    Agata de Sena L, Sanmartin de Almeida M, de Oliveira Fernandes V, Guerra Bretana R, Castro-Silva I, Granjeiro J, et al. Biocompatibility of wollastonite-poly(N-butyl-2-cyanoacrylate) composites. J Biomed Mater Res. 2014;102B:1121–9.CrossRefGoogle Scholar
  15. 15.
    Yang Y, Zhang L, Yang G, Gao C, Gou Z. Preparation and characterization of multi-shell hollow biphase bioceramic microsphere composites. J Inorg Mater. 2014;29:650–6.Google Scholar
  16. 16.
    Zhang W, Shen Y, Pan H, Lin K, Liu X, Darvell BW, et al. Effects of strontium in modified biomaterials. Acta Biomater. 2011;7:800–8.CrossRefGoogle Scholar
  17. 17.
    Lai W, Garino J, Flaitz C, Ducheyne P. Excretion of resorption products from bioactive glass implanted in rabbit muscle. J Biomed Mater Res. 2005;75A:398–407.CrossRefGoogle Scholar
  18. 18.
    Lai W, Garino J, Ducheyne P. Silicon excretion from bioactive glass implanted in rabbit bone. Biomaterials. 2002;23:213–7.CrossRefGoogle Scholar
  19. 19.
    Schwarz K. A bound form of silicon in glycosaminoglycans and polyuronides. Proc Natl Acad Sci USA. 1973;70:1608–12.CrossRefGoogle Scholar
  20. 20.
    Dobbie J, Smith M. The silicon content of body fluids. Scott Med J. 1982;27:17–9.Google Scholar
  21. 21.
    Hamilton E, Minski M, Cleary J. The concentration and distribution of some stable elements in healthy human tissues from the United Kingdom: an environmental study. Sci Total Environ. 1973;1:341–74.CrossRefGoogle Scholar
  22. 22.
    LeVier R. Distribution of silicon in the adult rat and rhesus monkey. Bioinorg Chem. 1975;4:109–15.CrossRefGoogle Scholar
  23. 23.
    Kawanabe K, Yamamuro T, Nakamura T, Kotani S. Effects of injecting massive amounts of bioactive ceramics in mice. J Biomed Mater Res. 1991;25A:117–28.CrossRefGoogle Scholar
  24. 24.
    Kawanabe K, Yamamuro T, Kotani S, Nakamura T. Acute nephrotoxicity as an adverse effect after intraperitoneal injection of massive amounts of bioactive ceramic powders in mice and rats. J Biomed Mater Res. 1992;26A:209–19.CrossRefGoogle Scholar
  25. 25.
    Nagase M, Abe Y, Chigira M, Udagawa E. Toxicity of silica containing calcium-phosphate glasses demonstrated in mice. Biomaterials. 1992;13:172–5.CrossRefGoogle Scholar
  26. 26.
    Lin K, Chang J, Zeng Y, Qian W. Preparation of macroporous calcium silicate ceramics. Mater Lett. 2004;58:2109–13.CrossRefGoogle Scholar
  27. 27.
    Maehira F, Miyagi I, Eguchi Y. Effects of calcium sources and soluble silicate on bone metabolism and the related gene expression in mice. Nutrition. 2009;25:581–9.CrossRefGoogle Scholar
  28. 28.
    Pietak AM, Reid JW, Stott MJ, Sayer M. Silicon substitution in the calcium phosphate bioceramics. Biomaterials. 2007;28:4023–32.CrossRefGoogle Scholar
  29. 29.
    Hoppe A, Guldal NS, Boccaccini AR. A review of the biological response to ionic dissolution products from bioactive glasses and glass-ceramics. Biomaterials. 2011;32:2757–74.CrossRefGoogle Scholar
  30. 30.
    Zhai Q, Zhao S, Wang X, Li X, Li W, Zheng Y. Synthesis and characterization of bionic nano-silicon-substituted hydroxyapatite. J Inorg Mater. 2013;28:58–62.CrossRefGoogle Scholar
  31. 31.
    D€orfler A, Detsch R, Romeis S, Schmidt J, Eisermann C, Peukert W, et al. Biocompatibility of submicron Bioglass® powders obtained by a top-down approach. J Biomed Mater Res. 2014;102B:952–61.CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  1. 1.State Key Laboratory of High Performance Ceramics and Superfine Microstructure, Shanghai Institute of CeramicsChinese Academy of SciencesShanghaiChina
  2. 2.Institute of Orthopedic Surgery, Xijing HospitalFourth Military Medical UniversityXi’anChina

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