Degradation and silicon excretion of the calcium silicate bioactive ceramics during bone regeneration using rabbit femur defect model

  • Kaili Lin
  • Yong Liu
  • Hai Huang
  • Lei Chen
  • Zhen WangEmail author
  • Jiang ChangEmail author
Biocompatibility Studies
Part of the following topical collections:
  1. Biocompatibility Studies


The investigation of the bone regeneration ability, degradation and excretion of the grafts is critical for development and application of the newly developed biomaterials. Herein, the in vivo bone-regeneration, biodegradation and silicon (Si) excretion of the new type calcium silicate (CaSiO3, CS) bioactive ceramics were investigated using rabbit femur defect model, and the results were compared with the traditional β-tricalcium phosphate [β-Ca3(PO4)2, β-TCP] bioceramics. After implantation of the scaffolds in rabbit femur defects for 4, 8 and 12 weeks, the bone regenerative capacity and degradation were evaluated by histomorphometric analysis. While urine and some organs such as kidney, liver, lung and spleen were resected for chemical analysis to determine the excretion of the ionic products from CS implants. The histomorphometric analysis showed that the bioresorption rate of CS was similar to that of β-TCP in femur defect model, while the CS grafts could significantly stimulate bone formation capacity as compared with β-TCP bioceramics (P < 0.05). The chemical analysis results showed that Si concentration in urinary of the CS group was apparently higher than that in control group of β-TCP. However, no significant increase of the Si excretion was found in the organs including kidney, which suggests that the resorbed Si element is harmlessly excreted in soluble form via the urine. The present studies show that the CS ceramics can be used as safe, bioactive and biodegradable materials for hard tissue repair and tissue engineering applications.


Calcium Silicate Silicon Concentration Calcium Silicate Calvarial Defect Implantation Period 
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This work was supported by grants from the Natural Science Foundation of China (Grant No.: 30900299, 81190132 and 51061160499).


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© Springer Science+Business Media New York 2015

Authors and Affiliations

  1. 1.State Key Laboratory of High Performance Ceramics and Superfine Microstructure, Shanghai Institute of CeramicsChinese Academy of SciencesShanghaiChina
  2. 2.Institute of Orthopedic Surgery, Xijing HospitalFourth Military Medical UniversityXi’anChina

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