In situ forming hydrogel composed of hyaluronate and polygalacturonic acid for prevention of peridural fibrosis

  • Cheng-Yi Lin
  • Hsiu-Hui Peng
  • Mei-Hsiu Chen
  • Jui-Sheng Sun
  • Tse-Ying LiuEmail author
  • Ming-Hong ChenEmail author
Clinical Applications of Biomaterials
Part of the following topical collections:
  1. Clinical Applications of Biomaterials


Hyaluronic acid-based hydrogels can reduce postoperative adhesion. However, the long-term application of hyaluronic acid is limited by tissue mediated enzymatic degradation. To overcome this limitation, we developed a polygalacturonic acid and hyaluronate composite hydrogel by Schiff’s base crosslinking reaction. The polygalacturonic acid and hyaluronate composite hydrogels had short gelation time (less than 15 s) and degraded by less than 50 % in the presence of hyaluronidase for 7 days. Cell adhesion and migration assays showed polygalacturonic acid and hyaluronate composite hydrogels prevented fibroblasts from adhesion and infiltration into the hydrogels. Compared to hyaluronate hydrogels and commercial Medishield™ gels, polygalacturonic acid and hyaluronate composite hydrogel was not totally degraded in vivo after 4 weeks. In the rat laminectomy model, polygalacturonic acid and hyaluronate composite hydrogel also had better adhesion grade and smaller mean area of fibrous tissue formation over the saline control and hyaluronate hydrogel groups. Polygalacturonic acid and hyaluronate composite hydrogel is a system that can be easy to use due to its in situ cross-linkable property and potentially promising for adhesion prevention in spine surgeries.


Hyaluronic Acid Gelation Time Compressive Modulus Composite Hydrogel Polygalacturonic Acid 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



Support for this research was provided through National Taiwan University Hospital Hsin-Chu Branch from Grants HCH 101-19.


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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  1. 1.Department of Biomedical EngineeringNational Yang-Ming UniversityTaipeiTaiwan, ROC
  2. 2.Department of MedicineFar Eastern Memory HospitalNew Taipei CityTaiwan
  3. 3.Department of OrthopedicsNational Taiwan University Hospital Hsin-Chu BranchHsinchuTaiwan
  4. 4.Division of Neurosurgery, Department of SurgeryTaipei Tzu Chi HospitalNew Taipei CityTaiwan
  5. 5.Department of Surgery, School of MedicineTzu Chi UniversityHualien CityTaiwan
  6. 6.Department of Biomedical EngineeringMing Chuang UniversityTaipeiTaiwan

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