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Chitosan and composite microsphere-based scaffold for bone tissue engineering: evaluation of tricalcium phosphate content influence on physical and biological properties

  • Martyna Kucharska
  • Katarzyna Walenko
  • Małgorzata Lewandowska-Szumieł
  • Tomasz Brynk
  • Jakub Jaroszewicz
  • Tomasz Ciach
Tissue Engineering Constructs and Cell Substrates
Part of the following topical collections:
  1. Tissue Engineering Constructs and Cell Substrates

Abstract

In the hereby presented work the authors describe a technique of high-compression-resistant biodegradable bone scaffold preparation. The methodology is based on the agglomeration of chitosan (CH) and chitosan/β-tricalcium phosphate (CH/TCP) microspheres and represents a novel approach to 3D matrices design for bone tissue engineering application. The materials were prepared from high deacetylation degree chitosan. The authors describe the method for scaffold fabrication, essential properties of the materials manufactured and the influence of various TCP concentrations on material morphology, mechanical properties (for dry and hydrated materials) and preliminary study on the interaction between CH or CH/TCP scaffolds and within cultured MG-63 osteoblast-like cells. The properties of the obtained materials were significantly affected by the calcium phosphate content, which had a particular influence on the granule microstructure, size distribution and inner biomaterial pore size. The water uptake ability was found to be lower for the materials enriched with the inorganic phase and tended to decrease with the increasing calcium phosphate concentration. The evaluation of mechanical properties has revealed that scaffolds produced with the usage of granule-based technology display a potential to be used as a load-bearing material since the Young’s modulus values were limited to the range of 200–500 MPa for dry materials and 15–20 MPa for the hydrated state of the scaffolds. The cell number, identified in three time points (48 h, 7 and 14 days) by Pico Green assay, was lower for the materials enriched with inorganic phase (75 % of control), however cell distribution, when compared to CH only biomaterial, was acknowledged as steadier on the surface of the material containing the highest calcium phosphate concentration.

Keywords

Chitosan Calcium Phosphate Bone Tissue Engineering Biphasic Calcium Phosphate Inorganic Phase 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

This work has been supported by the European Union in the framework of European Social Fund through the Warsaw University of Technology Development Programme1# and Grant No. NR 13-0008-10/2010 from The National Centre for Research and Development.

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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  • Martyna Kucharska
    • 1
    • 2
  • Katarzyna Walenko
    • 3
  • Małgorzata Lewandowska-Szumieł
    • 3
  • Tomasz Brynk
    • 4
  • Jakub Jaroszewicz
    • 4
  • Tomasz Ciach
    • 1
  1. 1.Biomedical Engineering Laboratory, Faculty of Chemical and Process EngineeringWarsaw University of TechnologyWarsawPoland
  2. 2.Polish Academy of ScienceInstitute of Fundamental Technological ResearchWarsawPoland
  3. 3.Department of Biophysics and Human PhysiologyMedical University of WarsawWarsawPoland
  4. 4.Faculty of Materials Science and EngineeringWarsaw University of TechnologyWarsawPoland

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